CITAB/Departamento de Biologia-Escola de Ciêkncias, Universidade do Minho (DB-ECUM), Campus de Gualtar, Braga, Portugal.
Int J Phytoremediation. 2011 Nov-Dec;13(10):947-58. doi: 10.1080/15226514.2010.532182.
Microbial monitoring of constructed wetlands (CWs) treating domestic wastewater is generally scarce, despite the need of more knowledge about its biocenosis. The sanitation quality of a wastewater treated in a CW is a crucial aspect, mainly when the receiving water body is used as a swimming and/or recreation area. The present study was carried out in a horizontal subsurface flow CWplanted with Phragmites australis receiving pre-treated domestic wastewater (mean flow 50 m3 day(-1)), from a population of about 300 inhabitants. The monitoring programme undertaken during the first year operation, revealed removal efficiencies of 61% BOD5, 44% COD, and 65% TSS for inlet water with ca. 90 mg L(-1) BOD5, 157 mg L(-1) COD, and 17 mg L(-1) TSS. Total Coliform (TC) and Faecal Coliform (FC) bacteria were removed from wastewater (mean inlet values of 5 x 10(6) CFU 100 mL(-1) TC and of 9 x 10(5) CFU 100 mL(-1) FC), with efficiencies of 92 and 97%, respectively. The dynamics of microbial communities established in the system assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), had revealed a high bacterial diversity within the system, with no relevant differences in composition at the CW inlet and outlet but exhibiting temporal differences in bacterial communities.
人工湿地（CWs）处理生活污水的微生物监测通常很少，尽管需要更多关于其生物群落的知识。CW 处理后的废水的卫生质量是一个关键方面，特别是当接收水体被用作游泳和/或娱乐区时。本研究在一个水平潜流 CW 中进行，该 CW 种植了芦苇（Phragmites australis），接收经过预处理的生活污水（平均流量为 50 立方米/天），来自约 300 名居民的人口。在第一年运行期间进行的监测计划表明，对于进水 BOD5 约为 90mg/L、COD 为 157mg/L、TSS 为 17mg/L 的情况，BOD5 的去除效率为 61%、COD 为 44%、TSS 为 65%。总大肠菌群（TC）和粪大肠菌群（FC）从废水中去除（进水平均值分别为 5 x 10(6) CFU 100 mL(-1) TC 和 9 x 10(5) CFU 100 mL(-1) FC），去除效率分别为 92%和 97%。通过聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）评估系统中建立的微生物群落的动态，发现系统内具有很高的细菌多样性，在 CW 入口和出口处的组成没有明显差异，但在细菌群落上存在时间差异。
