Health and Environmental Microbiology Laboratory, Optics and Optometry Department & AQUASOST - UNESCO Chair on Sustainability, Universitat Politècnica de Catalunya, Edifici Gaia, Pg. Ernest Lluch/Rambla Sant Nebridi, Terrassa 08222, Spain.
Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Water Res. 2014 May 15;55:304-12. doi: 10.1016/j.watres.2014.02.011. Epub 2014 Feb 14.
The prokaryotic microbial communities (Bacteria and Archaea) of three different systems operating in Denmark for the treatment of domestic wastewater (horizontal flow constructed wetlands (HFCW), vertical flow constructed wetlands (VFCW) and biofilters (BF)) was analysed using endpoint PCR followed by Denaturing Gradient Gel Electrophoresis (DGGE). Further sequencing of the most representative bacterial bands revealed that diverse and distinct bacterial communities were found in each system unit, being γ-Proteobacteria and Bacteroidetes present mainly in all of them, while Firmicutes was observed in HFCW and BF. Members of the Actinobacteria group, although found in HFCW and VFCW, seemed to be more abundant in BF units. Finally, some representatives of α, β and δ-Proteobacteria, Acidobacteria and Chloroflexi were also retrieved from some samples. On the other hand, a lower archaeal diversity was found in comparison with the bacterial population. Cluster analysis of the DGGE bacterial band patterns showed that community structure was related to the design of the treatment system and the organic matter load, while no clear relation was established between the microbial assemblage and the wastewater influent.
采用末端 PCR 结合变性梯度凝胶电泳 (DGGE) 技术分析了丹麦三个不同污水处理系统（水平流人工湿地 (HFCW)、垂直流人工湿地 (VFCW) 和生物滤池 (BF)) 中的原核微生物群落（细菌和古菌）。对最具代表性的细菌条带进行进一步测序表明，每个系统单元中都存在多样且独特的细菌群落，其中 γ-变形菌和拟杆菌门主要存在于所有系统单元中，而厚壁菌门则存在于 HFCW 和 BF 中。放线菌门的成员虽然存在于 HFCW 和 VFCW 中，但在 BF 单元中似乎更为丰富。最后，从一些样品中还提取到了一些α、β和δ-变形菌、酸杆菌门和绿弯菌门的代表。另一方面，与细菌种群相比，古菌的多样性较低。DGGE 细菌条带模式的聚类分析表明，群落结构与处理系统的设计和有机物负荷有关，而微生物组合与废水进水之间没有建立明确的关系。
