Platform Technology and Science, Drug Metabolism and Pharmacokinetics, GlaxoSmithKline Research and Development, Ware, Hertfordshire, United Kingdom.
Anal Chem. 2011 Nov 15;83(22):8779-86. doi: 10.1021/ac202321q. Epub 2011 Oct 21.
A novel technique is presented that addresses the issue of how to apply internal standard (IS) to dried matrix spot (DMS) samples that allows the IS to integrate with the sample prior to extraction. The TouchSpray, a piezo electric spray system, from The Technology Partnership (TTP), was used to apply methanol containing IS to dried blood spot (DBS) samples. It is demonstrated that this method of IS application has the potential to work in practice, for use in quantitative determination of circulating exposures of pharmaceuticals in toxicokinetic and pharmacokinetic studies. Three different methods of IS application were compared: addition of IS to control blood prior to DBS sample preparation (control 1), incorporation into extraction solvent (control 2), and the novel use of TouchSpray technology (test). It is demonstrated that there was no significant difference in accuracy and precision data using these three techniques obtained using both manual extraction and direct elution.
提出了一种新的技术，解决了如何将内标（IS）应用于干燥基质斑点（DMS）样品的问题，该技术允许 IS 在提取之前与样品整合。来自 The Technology Partnership（TTP）的压电喷雾系统 TouchSpray 被用于将含有内标的甲醇应用于干燥血斑（DBS）样品。结果表明，这种内标应用方法在实际应用中具有潜力，可用于定量测定毒代动力学和药代动力学研究中药物的循环暴露。比较了三种不同的内标应用方法：在 DBS 样品制备前将 IS 添加到对照血液中（对照 1）、将 IS 掺入提取溶剂中（对照 2）以及使用 TouchSpray 技术的新方法（测试）。结果表明，使用这三种技术通过手动提取和直接洗脱获得的准确性和精密度数据没有显著差异。
