Wilhelm Abraham J, den Burger Jeroen C G, Swart Eleonora L
Department of Clinical Pharmacology and Pharmacy, VU University Medical Center, de Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands,
Clin Pharmacokinet. 2014 Nov;53(11):961-73. doi: 10.1007/s40262-014-0177-7.
This article discusses dried blood spot (DBS) sampling in therapeutic drug monitoring (TDM). The most important advantages of DBS sampling in TDM are the minimally invasive procedure of a finger prick (home sampling), the small volume (children), and the stability of the analyte. Many assays in DBS have been reported in the literature over the previous 5 years. These assays and their analytical techniques are reviewed here. Factors that may influence the accuracy and reproducibility of DBS methods are also discussed. Important issues are the correlation with plasma/serum concentrations and the influence of hematocrit on spot size and recovery. The different substrate materials are considered. DBS sampling can be a valid alternative to conventional venous sampling. However, patient correlation studies are indispensable to prove this. Promising developments are dried plasma spots using membrane and hematocrit correction using the potassium concentration.
本文讨论了治疗药物监测（TDM）中的干血斑（DBS）采样。DBS采样在TDM中的最重要优势在于手指采血（家庭采样）的微创性、小样本量（儿童适用）以及分析物的稳定性。在过去5年中，文献报道了许多DBS检测方法。本文对这些检测方法及其分析技术进行了综述。还讨论了可能影响DBS方法准确性和可重复性的因素。重要问题包括与血浆/血清浓度的相关性以及血细胞比容对血斑大小和回收率的影响。文中考虑了不同的底物材料。DBS采样可以是传统静脉采样的有效替代方法。然而，必须进行患者相关性研究来证明这一点。有前景的进展包括使用膜的干血浆斑以及利用钾浓度进行血细胞比容校正。
