用于定量测定人血中胍法辛的干血斑半自动直接洗脱法。
Semi-automated direct elution of dried blood spots for the quantitative determination of guanfacine in human blood.
作者信息
Li Yuanyuan, Henion Jack, Abbott Richard, Wang Phillip
机构信息
Advion Bioanalytical Labs, Ithaca, NY, USA.
出版信息
Bioanalysis. 2012 Jun;4(12):1445-56. doi: 10.4155/bio.12.111.
BACKGROUND
Direct analysis of dried blood spot (DBS) samples was investigated using a prototype semi-automated robotic device that allows the direct elution of sample spots from a DBS paper card to an online SPE cartridge. The eluted SPE samples were analyzed with high-performance LC-MS/MS.
RESULTS
A LLOQ of 0.01 ng/ml was achieved with a linear calibration range from 0.01 to 25 ng/ml. Optimal performance data were obtained from spotting the internal standard solution on the card before blood spotting. Internal standard addition from the system injector loop produced intra-assay inaccuracy of -9.0-7.3% and precision of 1.3-8.2%, and inter-assay inaccuracy of -3.5-3.9% and precision of 4.4-8.7%.
CONCLUSION
Results demonstrated the feasibility of a semi-automated online rapid direct elution method that avoids manual extraction for DBS sample analysis using the online DBS-SPE system coupled to LC-MS/MS.
背景
使用一种原型半自动机器人设备对干血斑（DBS）样本进行直接分析，该设备可将DBS纸卡上的样本斑点直接洗脱至在线固相萃取（SPE）小柱。对洗脱后的SPE样本进行高效液相色谱-串联质谱（LC-MS/MS）分析。
结果
实现了0.01 ng/ml的定量下限，线性校准范围为0.01至25 ng/ml。在血样点样前将内标溶液点在卡上可获得最佳性能数据。通过系统进样环添加内标，批内不准确度为-9.0-7.3%，精密度为1.3-8.2%；批间不准确度为-3.5-3.9%，精密度为4.4-8.7%。
结论
结果证明了一种半自动在线快速直接洗脱方法的可行性，该方法避免了使用与LC-MS/MS联用的在线DBS-SPE系统对DBS样本分析进行手动提取。
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