猫白血病病毒群特异性抗原免疫荧光测定法的改良
Modifications of the immunofluorescence assay for feline leukemia virus group-specific antigens.
作者信息
Hoover E A, Mathes L E, Rojko J L, Schaller J P, Olsen R G
出版信息
Am J Vet Res. 1978 Dec;39(12):1877-80.
Observations and minor modifications are presented concerning the immunofluorescence assay for feline leukemia virus (FeLV) group-specific antigens (GSA) in blood cells of cats. Data are given regarding absorption of goat FeLV GSA antiserum in vivo in cats, absorption of the antiserum in vitro with feline blood cells, and the comparative efficacy of various chemical fixatives in preservation of FeLV GSA for immunofluorescent staining. The best results were obtained with in vitro absorption of antiserum and methanol fixation of FeLV GSA in blood smears.
本文呈现了关于猫白血病病毒（FeLV）群特异性抗原（GSA）在猫血细胞中的免疫荧光测定的观察结果和微小改进。给出了关于山羊FeLV GSA抗血清在猫体内的吸收、用猫血细胞在体外对抗血清的吸收以及各种化学固定剂在保存用于免疫荧光染色的FeLV GSA方面的比较效果的数据。抗血清的体外吸收和血涂片中铁白血病病毒群特异性抗原的甲醇固定取得了最佳结果。
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