Takada H, Hirai H, Fujiwara T, Koga T, Ogawa T, Hamada S
Department of Oral Microbiology, Osaka University Faculty of Dentistry, Japan.
J Infect Dis. 1990 Aug;162(2):428-34. doi: 10.1093/infdis/162.2.428.
Lipopolysaccharides (LPS) (100 micrograms/mouse) from Bacteroides gingivalis elicited anaphylactoid reactions in N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) (100 micrograms)-primed C3H/HeN mice 0-48 h after MDP injection. The reaction resulted in death within 1 h when the LPS was injected 2-10 h after the MDP injection. LPS prepared from other Bacteroides and Salmonella species but not from others, including Escherichia coli, could induce the reaction. LPS-nonresponsive C3H/HeJ and C57BL/6 and C57BL/10 strains were also responsive to the reaction, while AKR, BALB/c, DBA/2, and ICR mice were not. By contrast, Bacteroides LPS exhibited weak lethal toxicity compared with E. coli LPS in galactosamine-sensitized C3H/HeN mice, while neither LPS had activity in C3H/HeJ mice. LPS from E. coli and Bacteroides intermedius induced serum tumor necrosis factor (TNF) activity in MDP-primed mice, except for C3H/HeJ mice; B. gingivalis LPS rarely induced TNF in C3H/HeN or C3H/HeJ mice, indicating no involvement of TNF in the anaphylactoid reaction. Serotonin could substitute for MDP for the reaction, and methysergide, a serotonin antagonist, inhibited the activity.
牙龈卟啉单胞菌的脂多糖（LPS）（100微克/只小鼠）在N - 乙酰胞壁酰 - L - 丙氨酰 - D - 异谷氨酰胺（MDP）（100微克）致敏的C3H/HeN小鼠中，于MDP注射后0 - 48小时引发类过敏反应。当在MDP注射后2 - 10小时注射LPS时，该反应会在1小时内导致死亡。从其他卟啉单胞菌属和沙门氏菌属制备的LPS能诱导该反应，而包括大肠杆菌在内的其他菌属制备的LPS则不能。LPS无反应性的C3H/HeJ、C57BL/6和C57BL/10品系对该反应也有反应，而AKR、BALB/c、DBA/2和ICR小鼠则无反应。相比之下，在半乳糖胺致敏的C3H/HeN小鼠中，与大肠杆菌LPS相比，卟啉单胞菌LPS的致死毒性较弱，而在C3H/HeJ小鼠中两种LPS均无活性。除C3H/HeJ小鼠外，大肠杆菌和中间卟啉单胞菌的LPS在MDP致敏的小鼠中诱导血清肿瘤坏死因子（TNF）活性；牙龈卟啉单胞菌LPS在C3H/HeN或C3H/HeJ小鼠中很少诱导TNF，表明TNF不参与类过敏反应。血清素可替代MDP引发该反应，而血清素拮抗剂麦角新碱可抑制该活性。
