Sekiguchi K, Nishiyama C, Morishima T, Yoshiike T, Tsuboi R, Ogawa H
Department of Dermatology, Nihon University School of Medicine.
Nihon Hifuka Gakkai Zasshi. 1990 Feb;100(2):215-7.
We have previously reported that T. rubrum produced a new type of extracellular proteinase in liquid medium containing bovine serum albumin. This enzyme had an optimal pH 4.5 for azocoll. In this paper, we studied about the relation between movement of acid or alkaline proteinase and environmental pH. In the beginning of cultivation, acid proteolytic activity increased radically, and 3 or 4 weeks later, it was decreased. In place of acid proteinase, alkaline proteinase which had an optimal pH 8.0 was increased gradually. During incubation of T. rubrum in bovine serum albumin medium which was adjusted to pH 6.0, the pH of medium was gradually increased to about pH 7.0 toward 4 weeks. The growth of T. rubrum increased until pH in medium was fixed to pH 7.0.
我们之前曾报道，红色毛癣菌在含有牛血清白蛋白的液体培养基中产生了一种新型的细胞外蛋白酶。这种酶作用于偶氮胶原的最适pH为4.5。在本文中，我们研究了酸性或碱性蛋白酶的活性与环境pH之间的关系。在培养初期，酸性蛋白水解活性急剧增加，3或4周后降低。取而代之的是，最适pH为8.0的碱性蛋白酶逐渐增加。在将pH值调至6.0的牛血清白蛋白培养基中培养红色毛癣菌的过程中，培养基的pH值在4周内逐渐升高至约pH 7.0。直到培养基中的pH值稳定在pH 7.0之前，红色毛癣菌的生长都在增加。
