白色念珠菌产生蛋白酶（CAPP）与其环境pH值的关系——与须癣毛癣菌病例的比较。
Relationship between Candida albicans producing proteinase (CAPP) and its environmental pH--comparison with a case of trichophyton mentagrophytes.
作者信息
Ko I J, Kim C W, Houh W, Tsuboi R, Matsuda K, Ogawa H
机构信息
Department of Dermatology, Catholic University Medical College, Seoul, Korea.
出版信息
J Korean Med Sci. 1987 Jun;2(2):97-101. doi: 10.3346/jkms.1987.2.2.97.
Candida albicans produced a karatinolytic proteinase (KPase) or C. albicans producing proteinase (CAPP), a proposed new term for this enzyme, and Trichophyton mentagrophytes also produced KPase when cultivated in liquid medium containing human stratum corneum (HSC) as the nitrogen source, but were unable to do so when cultivated in sabouraud dextrose broth. Purified KPase from the culture supernatants of C. albicans had a molecular weight of 42,000 and an optimum pH at 4.0. The KPase was found to belong to the carboxyl proteinases group and its activity was strongly inhibited by pepstatin. Both fungi were able to grow by secreting KPase which digested HSC for nutrients. KPase from both fungi had high activity in each optimum pH, such as weakly acidic pH on C. albicans and neutral pH on T. mentagrophytes to adapt their surrounding environment by changing the environmental pH into their own optimum pH.
白色念珠菌产生一种角蛋白分解蛋白酶（KPase）或产蛋白酶白色念珠菌（CAPP，该酶的一个新提出的术语），并且须癣毛癣菌在以人角质层（HSC）作为氮源的液体培养基中培养时也产生KPase，但在沙氏葡萄糖肉汤中培养时则无法产生。从白色念珠菌培养上清液中纯化得到的KPase分子量为42,000，最适pH为4.0。该KPase被发现属于羧基蛋白酶组，其活性受到胃蛋白酶抑制剂的强烈抑制。两种真菌都能够通过分泌消化HSC以获取营养的KPase来生长。两种真菌的KPase在各自的最适pH下都具有高活性，比如白色念珠菌在弱酸性pH下，须癣毛癣菌在中性pH下，通过将环境pH改变为自身的最适pH来适应周围环境。
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