Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto City, Kumamoto 860-8555, Japan.
Bioresour Technol. 2011 Dec;102(23):10929-35. doi: 10.1016/j.biortech.2011.09.071. Epub 2011 Sep 22.
An efficient process for the production of fuel ethanol from bamboo that consisted of hydrolysis with concentrated sulfuric acid, removal of color compounds, separation of acid and sugar, hydrolysis of oligosaccharides and subsequent continuous ethanol fermentation was developed. The highest sugar recovery efficiency was 81.6% when concentrated sulfuric acid hydrolysis was carried out under the optimum conditions. Continuous separation of acid from the saccharified liquid after removal of color compounds with activated carbon was conducted using an improved simulated moving bed (ISMB) system, and 98.4% of sugar and 90.5% of acid were recovered. After oligosaccharide hydrolysis and pH adjustment, the unsterilized saccharified liquid was subjected to continuous ethanol fermentation using Saccharomycescerevisiae strain KF-7. The ethanol concentration, the fermentation yield based on glucose and the ethanol productivity were approximately 27.2 g/l, 92.0% and 8.2 g/l/h, respectively. These results suggest that the process is effective for production of fuel ethanol from bamboo.
从竹子中生产燃料乙醇的高效工艺包括浓H2SO4水解、去除色素、酸和糖的分离、低聚糖水解以及随后的连续乙醇发酵。在最佳条件下进行浓 H2SO4水解时，糖回收率最高可达 81.6%。在去除色素后，使用改进的模拟移动床（ISMB）系统连续从糖化液中分离酸，可回收 98.4%的糖和 90.5%的酸。低聚糖水解和 pH 调整后，未经灭菌的糖化液用酿酒酵母 KF-7 进行连续乙醇发酵。乙醇浓度、基于葡萄糖的发酵产率和乙醇生产率分别约为 27.2 g/L、92.0%和 8.2 g/L/h。这些结果表明，该工艺可有效从竹子中生产燃料乙醇。
