State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Bioresour Technol. 2010 Jul;101(13):4959-64. doi: 10.1016/j.biortech.2009.11.010. Epub 2009 Dec 9.
To obtain high concentration of ethanol from cellulose, corncob was pretreated with acid and alkali to remove non-cellulose components, and then subjected to simultaneous saccharification and fermentation (SSF). An ethanol concentration as high as 69.2 g/L was achieved with 19% dry matter (DM) using batch SSF, resulting in an 81.2% overall ethanol yield. A fed-batch process using a high solid concentration was also investigated. Fresh substrate was pretreated with dilute sulfuric acid-sodium hydroxide, and then added at different amounts during the first 24 h, to yield a final dry matter content of 25% (w/v). SSF conditions with cellulose loading of 22.8 FPU/g glucan, dry yeast (Saccharomyces cerevisiae) loading of 5 g/L and substrate supplementation every 4h yielded the highest ethanol concentration of 84.7 g/L after 96 h. This corresponded to a 79% overall ethanol yield.
为了从纤维素中获得高浓度的乙醇，采用酸碱预处理玉米芯以去除非纤维素成分，然后进行同步糖化发酵（SSF）。采用分批 SSF，用 19%的干物质（DM）实现了高达 69.2g/L 的乙醇浓度，总乙醇得率为 81.2%。还研究了使用高固体浓度的分批补料工艺。新鲜底物用稀硫酸-氢氧化钠预处理，然后在前 24 小时内以不同的量添加，最终干物质含量为 25%（w/v）。在纤维素负荷为 22.8FPU/g 葡聚糖、干酵母（酿酒酵母）负荷为 5g/L 的 SSF 条件下，每 4 小时补充底物，96 小时后可获得最高 84.7g/L 的乙醇浓度，总乙醇得率为 79%。
