Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, Uttrakhand, India.
Bioresour Technol. 2011 Nov;102(21):9970-5. doi: 10.1016/j.biortech.2011.08.023. Epub 2011 Aug 10.
Bioethanol is one of the alternatives of the conventional fossil fuel. In present study, effect of different carbon sources on the production of cellulolytic enzyme (CMCase) from Trichoderma reesei at different temperatures, duration and pH were investigated and conditions were optimized. Acid treated Kans grass (Saccharum sponteneum) was subjected to enzymatic hydrolysis to produce fermentable sugars which was then fermented to bioethanol using Saccharomyces cerevisiae. The maximum CMCase production was found to be 1.46 U mL(-1) at optimum condition (28°C, pH 5 and cellulose as carbon source). The cellulases and xylanase activity were found to be 1.12 FPU g(-1) and 6.63 U mL(-1), respectively. Maximum total sugar was found to be 69.08 mg/g dry biomass with 20 FPU g(-1) dry biomass of enzyme dosage under optimum condition. Similar results were obtained when it was treated with pure enzyme. Upon fermentation of enzymatic hydrolysate, the yield of ethanol was calculated to be 0.46 g g(-1).
生物乙醇是传统化石燃料的替代品之一。在本研究中，考察了不同碳源在不同温度、时间和 pH 值条件下对里氏木霉产纤维素酶（CMCase）的影响，并对条件进行了优化。酸处理后的肯氏草（Saccharum sponteneum）进行酶解以产生可发酵糖，然后使用酿酒酵母发酵成生物乙醇。在最佳条件（28°C、pH5 和纤维素作为碳源）下，CMCase 的最大产量为 1.46 U mL(-1)。纤维素酶和木聚糖酶的活性分别为 1.12 FPU g(-1)和 6.63 U mL(-1)。在最佳条件下，酶用量为 20 FPU g(-1)干生物质时，最大总糖含量为 69.08 mg/g 干生物质。用纯酶处理时也得到了类似的结果。对酶解液进行发酵后，计算出乙醇的得率为 0.46 g g(-1)。
