Paroul Natália, Grzegozeski Luana Paula, Chiaradia Viviane, Treichel Helen, Cansian Rogério L, Oliveira J Vladimir, de Oliveira Débora
Programa de Pós-Graduação em Biotecnologia, Universidade de Caxias do Sul, Caxias do Sul, Rio Grande do Sul, Brazil.
Universidade Regional Integrada do Alto Uruguai e das Missões, URI-Campus de Erechim, Av. Sete de Setembro, 1621, 99700-000, Erechim, Rio Grande do Sul, Brazil.
Appl Biochem Biotechnol. 2012 Jan;166(1):13-21. doi: 10.1007/s12010-011-9399-4. Epub 2011 Oct 6.
Enzymatic esterification of citronella essential oil towards the production of geranyl and citronellyl esters may present great scientific and technological interest due to the well-known drawbacks of the chemical-catalyzed route. In this context, this work reports the maximization of geranyl and citronellyl esters production by esterification of oleic and propionic acids in a solvent-free system using a commercial immobilized lipase as catalyst. Results of the reactions showed that the strategy adopted for the experimental design proved to be useful in evaluating the effects of the studied variables on the reaction conversion using Novozym 435 as catalyst. The operating conditions that maximized the production of each ester were determined, leading, in a general way, to conversions of about 90% for all systems. New experimental data on enzymatic esterification of crude citronella essential oil for geranyl and citronellyl esters production in solvent-free system are reported in this work.
由于化学催化路线存在众所周知的缺点，香茅精油的酶促酯化反应以生产香叶酯和香茅酯可能具有重大的科学和技术意义。在此背景下，本工作报道了在无溶剂体系中，以商业固定化脂肪酶为催化剂，通过油酸和丙酸的酯化反应使香叶酯和香茅酯产量最大化。反应结果表明，所采用的实验设计策略被证明对于评估所研究变量对使用诺维信435作为催化剂的反应转化率的影响是有用的。确定了使每种酯产量最大化的操作条件，总体而言，所有体系的转化率约为90%。本工作报道了在无溶剂体系中粗香茅精油酶促酯化生产香叶酯和香茅酯的新实验数据。
