多重 PCR 方法检测艰难梭菌 tcdA、tcdB、cdtA、cdtB 和 tcdC 内部框内缺失。
Multiplex PCR method for detection of Clostridium difficile tcdA, tcdB, cdtA, and cdtB and internal in-frame deletion of tcdC.
机构信息
Department of Microbiological Diagnostics, The National Reference Laboratory for Enteropathogenic Bacteria, Unit of Gastrointestinal Infections, Statens Serum Institut, Ørestads Boulevard 5, 2300 Copenhagen S, Denmark.
出版信息
J Clin Microbiol. 2011 Dec;49(12):4299-300. doi: 10.1128/JCM.05161-11. Epub 2011 Oct 5.
Abstract
A multiplex PCR method was developed for the detection of Clostridium difficile toxin genes tcdA, tcdB, ctdA, and cdtB and the major in-frame deletion types (18, 39, and 54 bp) of tcdC. The method has high specificity for PCR ribotype 027 and may identify other C. difficile strains of clinical and epidemiological importance.
摘要
建立了一种多重 PCR 方法，用于检测艰难梭菌毒素基因 tcdA、tcdB、ctdA 和 cdtB 以及 tcdC 的主要框内缺失类型（18、39 和 54 bp）。该方法对 PCR 核糖型 027 具有高度特异性，并且可以鉴定其他具有临床和流行病学意义的艰难梭菌菌株。
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