Department of Genetics and Molecular Diagnostics, Liberec Regional Hospital, 46001, Liberec, Czech Republic.
Department of Microbiology and Immunology, Liberec Regional Hospital, 46001, Liberec, Czech Republic.
Folia Microbiol (Praha). 2023 Apr;68(2):315-320. doi: 10.1007/s12223-022-01021-z. Epub 2022 Dec 1.
The ribotyping of Clostridioides difficile is one of the basic methods of molecular epidemiology for monitoring the spread of C. difficile infections. In the Czech Republic, this procedure is mainly available in university hospitals. The introduction of ribotyping in a tertiary health care facility such as Liberec Regional Hospital not only increases safety in the facility but also supports regional professional development. In our study, 556 stool samples collected between June 2017 and June 2018 were used for C. difficile infection screening, followed by cultivation, toxinotyping, and ribotyping of positive samples. The toxinotyping of 96 samples revealed that 44.8% of typed strains could produce toxins A and B encoded by tcdA and tcdB, respectively. The ribotyping of the same samples revealed two epidemic peaks, caused by the regionally most prevalent ribotype 176 (n = 30, 31.3). C. difficile infection incidence ranged between 5.5 and 4.2 cases per 10,000 patient-bed days. Molecular diagnostics and molecular epidemiology are the two most developing parts of clinical laboratories. The correct applications of molecular methods help ensure greater safety in hospitals.
艰难梭菌的核糖体分型是监测艰难梭菌感染传播的分子流行病学的基本方法之一。在捷克共和国，该程序主要在大学医院中提供。在利贝雷茨地区医院这样的三级医疗机构中引入核糖体分型不仅可以提高设施的安全性，还可以支持区域专业发展。在我们的研究中，使用了 2017 年 6 月至 2018 年 6 月之间收集的 556 份粪便样本进行艰难梭菌感染筛查，随后对阳性样本进行培养、毒素分型和核糖体分型。对 96 份样本的毒素分型表明，有 44.8%的定型菌株可以分别产生由 tcdA 和 tcdB 编码的毒素 A 和 B。对相同样本的核糖体分型显示出两个流行高峰，分别由区域内最流行的核糖体型 176（n=30，31.3%）引起。艰难梭菌感染的发病率在每 10000 个患者床日之间为 5.5 至 4.2 例。分子诊断和分子流行病学是临床实验室中发展最快的两个部分。正确应用分子方法有助于确保医院的更高安全性。
