Clifton I J, Denton M, M'zali F, Peckham D G
Regional Adult Cystic Fibrosis Unit, St James University Hospital, Leeds LS9 7TF, UK.
Int J Microbiol. 2011;2011:712618. doi: 10.1155/2011/712618. Epub 2011 Sep 29.
Prior to modern typing methods, cross-infection of P. aeruginosa between people with cystic fibrosis (CF) was felt to be rare. Recently a number of studies have demonstrated the presence of clonal strains of P. aeruginosa infecting people with CF. The aim of this study was to determine whether strains of P. aeruginosa demonstrated differences in resistance to desiccation and whether preincubation in subminimum inhibitory concentrations (MICs) of β-lactam affected desiccation resistance. The experimental data were modelled to a first-order decay model and a Weibull decay model using least squares nonlinear regression. The Weibull model was the preferred model for the desiccation survival. The presence of a mucoid phenotype promoted desiccation survival. Preincubation with antibiotics did not have a consistent effect on the strains of P. aeruginosa. Meropenem reduced desiccation resistance, whereas ceftazidime had much less effect on the strains studied.
在现代分型方法出现之前，人们认为囊性纤维化（CF）患者之间铜绿假单胞菌的交叉感染很少见。最近，一些研究已经证明存在感染CF患者的铜绿假单胞菌克隆菌株。本研究的目的是确定铜绿假单胞菌菌株在抗干燥能力上是否存在差异，以及在低于最低抑菌浓度（MIC）的β-内酰胺类药物中预孵育是否会影响抗干燥能力。使用最小二乘非线性回归将实验数据拟合为一级衰变模型和威布尔衰变模型。威布尔模型是描述干燥存活情况的首选模型。黏液样表型的存在促进了干燥存活。用抗生素预孵育对铜绿假单胞菌菌株没有一致的影响。美罗培南降低了抗干燥能力，而头孢他啶对所研究的菌株影响小得多。
