Malcolm Kenneth C, Caceres Silvia M, Honda Jennifer R, Davidson Rebecca M, Epperson L Elaine, Strong Michael, Chan Edward D, Nick Jerry A
Department of Medicine, National Jewish Health, Denver, Colorado, USA
Department of Medicine, University of Colorado Denver, Aurora, Colorado, USA.
Appl Environ Microbiol. 2017 Sep 15;83(19). doi: 10.1128/AEM.00562-17. Print 2017 Oct 1.
is a rapidly growing nontuberculous mycobacterium (NTM) increasingly reported in soft tissue infections and chronic lung diseases, including cystic fibrosis. The environmental source of has not been definitively identified, but NTM have been detected in soil and water. To determine the potential of soil-derived as an infectious source, we explored the association, growth, and survival of with defined mineral particulates, including kaolin, halloysite, and silicone dioxide, and house dust as possible fomites. physically associated with particulates, and the growth of was enhanced in the presence of both kaolin and house dust. survived desiccation for 2 weeks but was not viable after 3 weeks. The rate of decline of viability during desiccation was reduced in the presence of house dust. The evidence for enhanced growth and survival of during alternating growth and drying periods suggests that dissemination could occur when in wet or dry environments. These studies are important to understand environmental survival and acquisition of NTM. The environmental source of pulmonary infections is not known. Fomites are nonliving carriers of infectious agents and may contribute to acquisition of This study provides evidence that growth is enhanced in the presence of particulates, using kaolin, an abundant natural clay mineral, and house dust as experimental fomites. Moreover, survived desiccation for up to 2 weeks in the presence of house dust, kaolin, and several chemically defined mineral particulates; mycobacterial viability during extended periods of dessication was enhanced by the presence of house dust. The growth characteristics of with particulates suggest that a fomite mechanism of transmission may contribute to acquisition, which may lead to strategies to better control infections by and related organisms.
是一种快速增长的非结核分枝杆菌（NTM），在软组织感染和慢性肺部疾病（包括囊性纤维化）中报道日益增多。的环境来源尚未明确确定，但在土壤和水中已检测到NTM。为了确定土壤来源的作为传染源的可能性，我们探索了与特定矿物颗粒（包括高岭土、埃洛石和二氧化硅）以及房屋灰尘作为可能的污染物之间的关联、生长和存活情况。与颗粒发生物理关联，并且在高岭土和房屋灰尘同时存在的情况下的生长得到增强。在干燥环境中存活了2周，但3周后不再存活。在房屋灰尘存在的情况下，干燥过程中活力下降的速率降低。在交替的生长和干燥期生长和存活增强的证据表明，在潮湿或干燥环境中都可能发生传播。这些研究对于理解NTM的环境存活和感染获取很重要。肺部感染的环境来源尚不清楚。污染物是传染病原体的无生命载体，可能有助于感染的获取。本研究提供了证据，表明使用丰富的天然粘土矿物高岭土和房屋灰尘作为实验污染物时，在颗粒存在的情况下生长得到增强。此外，在房屋灰尘、高岭土和几种化学定义的矿物颗粒存在的情况下，在干燥环境中存活长达2周；房屋灰尘的存在增强了长时间干燥期间的分枝杆菌活力。与颗粒的生长特征表明，污染物传播机制可能有助于感染的获取，这可能导致更好地控制感染及相关生物体的策略。
