Department of Therapeutic Chemistry and Infectious Diseases and Immunology Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, Dokki, Cairo, Egypt.
East Mediterr Health J. 2011 Aug;17(8):679-86.
Crude antigenic preparations from Setaria equina were used in ELISA and Western blotting to examine cross-reaction with human sera from areas endemic for bancroftian filariasis. Sera from normal subjects from non-endemic areas were included as negative controls. Cross-reaction was found between S. equina antigens and antibodies in the sera of Wuchereria bancrofti-infected patients, with the highest levels observed between sera of chronic infected patients and Setaria spp. crude female worm surface antigen (CFSWA). In the absence of active transmission of Setaria spp. infection, CFWSA is useful to detect chronic W. bancrofti infection before patients become symptomatic, particularly when chronic patients are known to be amicrofilaraemic. In the presence of active S. equina infection, antigens from the adult and microfilaraemic stages showed the highest degree of cross-reaction with human sera.
使用来自马氏蚋的粗抗原制剂，通过 ELISA 和 Western blot 法，检测与流行地区班氏丝虫病患者血清的交叉反应。将非流行地区的正常人群血清作为阴性对照。在感染班氏丝虫的患者血清中，发现了马氏蚋抗原与抗体之间的交叉反应，在慢性感染患者血清和马氏蚋粗雌虫表面抗原（CFSWA）之间观察到最高水平的反应。在没有马氏蚋感染的活动传播的情况下，CFSWA 可用于在患者出现症状之前检测慢性班氏丝虫感染，特别是当已知慢性患者为微丝蚴血症时。在存在马氏蚋成虫和微丝蚴阶段的感染时，这些阶段的抗原与人类血清的交叉反应程度最高。
