指状丝虫抗原：与班氏吴策线虫微丝蚴表面抗原及班氏吴策线虫感染患者血清抗体的交叉反应
Antigen of Setaria digitata: cross-reaction with surface antigens of Wuchereria bancrofti microfilariae and serum antibodies of W. bancrofti-infected subjects.
作者信息
Dissanayake S, Ismail M M
出版信息
Bull World Health Organ. 1980;58(4):649-54.
Abstract
Extracts of Setaria digitata have been fractionated by DEAE-Sephadex A50 chromatography and the fractions obtained were used in the inhibition of indirect immunofluorescence of Wuchereria bancrofti microfilariae. The fractions were also tested by an enzyme-linked immunosorbent assay (ELISA) technique for reactivity against antibodies in the serum of patients with W. bancrofti infections. The results indicate that S. digitata contains several antigenic fractions that show cross-reactivity against filariasis sera; two of these fractions cross-reacted with surface antigens of W. bancrofti microfilariae but, when tested by the ELISA technique, these antigens did not show the highest reactivity against serum antibodies.
摘要
狗尾草提取物已通过DEAE-葡聚糖A50色谱法进行分级分离，所得级分用于抑制班氏吴策线虫微丝蚴的间接免疫荧光。这些级分还通过酶联免疫吸附测定（ELISA）技术检测其与班氏吴策线虫感染患者血清中抗体的反应性。结果表明，狗尾草含有几种抗原级分，它们与丝虫病血清显示交叉反应；其中两个级分与班氏吴策线虫微丝蚴的表面抗原发生交叉反应，但在用ELISA技术检测时，这些抗原对血清抗体并未显示出最高反应性。
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