Zhao Peng, Zhang Xin, Kang Fu-Biao, Zhang Xin-Wei, Zhang Liu, Tan Wen-Hui, Chen Wei-Wei, Zhao Min
PLA Medical School for Advanced Study, Beijing 100853, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Jun;25(3):182-4.
To investigate the characteristics of inhibitory and activating receptor expressions on natural killer (NK) cells in HIV/HCV co-infected patients.
Numbers, frequencies and expressions of activating and inhibitory receptors of NK cells were measured with flow cytometry (FCS) from HIV/HCV co-infected group (n = 24), HCV mono-infected group (n = 34), HIV mono-infected group (n = 21) and healthy control group (HC, n = 20), then analysis and compare were performed among those groups.
The NK cell absolute counts in HIV/HCV group were significantly lower than those in other three groups. The NKP30 and NKP46 frequencies on NK cells in HIV/HCV, HIV and HCV groups were all significantly lower than those in HC group, but there were no significant differences of NKP30 among former three groups; and NKP46 frequencies in HIV/HCV and HIV groups were lower than those in HCV group, but there were no significant differences between former two groups. The NKG2A frequencies in HIV/HCV and HCV groups were all higher than those in HIV and HC groups significantly, but the NKG2A frequencies in HIV group were lower than those in HC group; There were no significant differences of NKG2D, CD158a and CD158b among those four groups.
NK cell numbers and expressions of activiting receptors on NK cells obviously decreased in HIV/HCV co-infected patients, but some inhibitory receptors expressions increased, even higher than those of HIV mono-infected patients. NK cells impairments in HIV/HCV co-infection is more severe than HIV or HCV mono-infection.
探讨人类免疫缺陷病毒（HIV）/丙型肝炎病毒（HCV）合并感染患者自然杀伤（NK）细胞上抑制性和激活性受体的表达特征。
采用流式细胞术（FCS）检测HIV/HCV合并感染组（n = 24）、HCV单感染组（n = 34）、HIV单感染组（n = 21）和健康对照组（HC，n = 20）中NK细胞的数量、频率以及激活性和抑制性受体的表达，然后对这些组进行分析和比较。
HIV/HCV组的NK细胞绝对计数显著低于其他三组。HIV/HCV组、HIV组和HCV组NK细胞上的NKP30和NKP46频率均显著低于HC组，但前三组之间NKP30无显著差异；HIV/HCV组和HIV组的NKP46频率低于HCV组，但前两组之间无显著差异。HIV/HCV组和HCV组的NKG2A频率均显著高于HIV组和HC组，但HIV组的NKG2A频率低于HC组；四组之间NKG2D、CD158a和CD158b无显著差异。
HIV/HCV合并感染患者的NK细胞数量和NK细胞上激活性受体的表达明显降低，但一些抑制性受体的表达增加，甚至高于HIV单感染患者。HIV/HCV合并感染时NK细胞的损伤比HIV或HCV单感染更严重。
