Zhang Xin, Zhao Peng, Kang Fu-biao, Zhang Xin-wei, Si Chan, Chen Xue-li, Chen Wei-wei, Zhao Min
Treatment and Research Center for Infectious Disease of the 302 Hospital of PLA, Beijing 100039, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Aug;25(4):248-50.
To study the impacts of HIV/HCV co-infection to NK cells by investigating the changes of frequencies and functions of NK cells in HIV/HCV co-infected patients.
Frequencies and counts of NK cells were measured in patients with HIV mono-infection, HCV monoinfection, HIV/HCV co-infection and health control (HC) group by flow cytometer (FCM). After stimulated with PMA and K562 cells, PBMCs were examined the proportion of IFN-gamma+ NK cells by FCM. Proportion of killed K562 cells were detected to analyze the killing functions of NK cells.
The frequencies of NK cells, the percentages of IFN-gamma+ NK cells as well as the functions of NK cells killing the K562 cells in HIV/HCV co-infection, HIV mono-infection and HCV mono-infection groups were all lower than those of HC group significantly, the absolute counts and killing functions of NK cells in co-infection group were significantly lower than those of HIV or HCV mono-infection group.
The counts and functions of NK cells were affected more in HIV/HCV co-infections than those in HIV or HCV mono-infection.
通过研究HIV/HCV合并感染患者自然杀伤细胞（NK细胞）频率和功能的变化，探讨HIV/HCV合并感染对NK细胞的影响。
采用流式细胞仪（FCM）检测HIV单感染、HCV单感染、HIV/HCV合并感染患者及健康对照组（HC组）中NK细胞的频率和计数。用佛波酯（PMA）和K562细胞刺激外周血单个核细胞（PBMCs）后，采用FCM检测IFN-γ+NK细胞的比例。检测K562细胞被杀伤的比例以分析NK细胞的杀伤功能。
HIV/HCV合并感染组、HIV单感染组和HCV单感染组中NK细胞的频率、IFN-γ+NK细胞的百分比以及NK细胞杀伤K562细胞的功能均显著低于HC组，合并感染组NK细胞的绝对计数和杀伤功能显著低于HIV或HCV单感染组。
与HIV或HCV单感染相比，HIV/HCV合并感染时NK细胞的计数和功能受到的影响更大。
