[乙型肝炎肝硬化患者病毒基因型与特异性及非特异性CTL的关系及其意义]

[Relationship between viral genotype and specific and nonspecific CTL of patients with cirrhotic hepatitis B and its significance].

作者信息

Gu Xi-Bing, Yang Xiao-Juan, Wang Juan-Hua, Hua Zhong, Lu Zhong-Hua, Xu Yue-Qin, Jia Zhi-Yuan

机构信息

Wuxi Hospital for Infectious Diseases, Wuxi 214005, China.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Jun;25(3):211-3.

PMID:21977595

Abstract

OBJECTIVE

To explore relationship between different HBV genotypes and peripheral blood HBV specific and nonspecific CTL of patients with cirrhotic hepatitis B and its significance.

METHODS

HBV genotypes were tested in 91 patients with cirrhotic hepatitis B, differences of HBV specific and nonspecific CTL between patients infected with genotype B and C were compared and its significance was explored.

RESULTS

In 91 cases of cirrhotic hepatitis B, 55 cases (60.44%) belong to genotype C, 35 cases (38.46%) belong to genotype B, 1 case (1.1%) belongs to mixture genotype B and C. In genotype C, 27 cases (49.09%) had positive (HLA)-A2, HBV specific CTL was 0.18% +/- 0.03%. In genotype B, 18 cases (51.43%) had positive HLA-A2, HBV specific CTL was 0.38% +/- 0.04%, higher than that in genotype C, t = 5.01, P < 0.01. Nonspecific CTL: genotype C (11.87% +/- 1.50%); genotype B (11.90% +/- 1.51%), t = 0.14, P < 0.05. HBV DNA level: genotype C (6.01 +/- 0.81) log10 copy/ml, higher than that in genotype B (5.01 +/- 0.54) log10 copy/ml, t = 5.01, P < 0.01. ALT: genotype C (251.13 +/- 131.11) U/L, higher than that in genotype B (121.25 +/- 63.21) U/L, t = 3.61, P < 0.01. TBil (45.61 +/- 15.11) micromol/L, higher than that in genotype B (28.11 +/- 6.25) micromol/L, t = 3.05, P < 0.01.

CONCLUSION

Compared with patients infected with genotype B of cirrhotic hepatitis B, HBV specific CTL of patients infected with genotype C was lower, resulting in higher level of HBV DNA and more severe damage of liver function.

摘要

目的

探讨不同乙肝病毒（HBV）基因型与乙型肝炎肝硬化患者外周血HBV特异性及非特异性细胞毒性T淋巴细胞（CTL）之间的关系及其意义。

方法

对91例乙型肝炎肝硬化患者进行HBV基因型检测，比较B基因型和C基因型感染者HBV特异性及非特异性CTL的差异，并探讨其意义。

结果

91例乙型肝炎肝硬化患者中，55例（60.44%）为C基因型，35例（38.46%）为B基因型，1例（1.1%）为B和C混合基因型。C基因型中，27例（49.09%）HLA - A2阳性，HBV特异性CTL为0.18%±0.03%。B基因型中，18例（51.43%）HLA - A2阳性，HBV特异性CTL为0.38%±0.04%，高于C基因型，t = 5.01，P < 0.01。非特异性CTL：C基因型（11.87%±1.50%）；B基因型（11.90%±1.51%），t = 0.14，P < 0.05。HBV DNA水平：C基因型（6.01±0.81）log10拷贝/ml，高于B基因型（5.01±0.54）log10拷贝/ml，t = 5.01，P < 0.01。谷丙转氨酶（ALT）：C基因型（251.13±131.11）U/L，高于B基因型（121.25±63.21）U/L，t = 3.61，P < 0.01。总胆红素（TBil）（45.61±15.11）μmol/L，高于B基因型（28.11±6.25）μmol/L，t = 3.05，P < 0.01。