Lubis Andri M T, Sandhow Lakshmi, Lubis Vita K, Noor Alfiah, Gumay Febrilian, Merlina Maurin, Yang Wenny, Kusnadi Yuyus, Lorensia Vinci, Sandra Ferry, Susanto Nugroho H
Department of Surgery, Faculty of Medicine, University of Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia.
Acta Med Indones. 2011 Jul;43(3):178-84.
to validate isolation and cultivation methods of bone marrow mesenchymal stem cells (BM-MSCs) from iliac crest, and to compare biological characteristics of BM-MSCs from different age groups for preparation of autologous stem cell therapy in cartilage defect.
patients undergoing spinal surgery were selected and grouped according to age. Iliac crest bone marrow from the patients was aspirated. BM-MSCs were isolated from the bone marrow and then cultivated. Their biological characteristics including morphological appearances and surface biomarkers were evaluated. Growth curves were observed. Sterility and Mycoplasma tests were also performed for quality assessment of BM-MSCs culture procedure.
in average, cultivated-BM-MSCs reached the number of 7.56-22.95 x 106 in 4-7 weeks period. BM-MSCs of all age groups showed the same quality of morphology, shape and surface biomarkers (CD105+, CD73+, CD34-, CD45-, CD14-, CD19-, HLA-DR-).
our procedures in isolating and cultivating of BM-MSCs have reached required amount for implantation into the cartilage lesion. In addition, the cultivated-BM-MSCs' biological characteristics were also in accordance with International Society of Cell Therapy (ISCT) MSCs criteria.
验证从髂嵴获取骨髓间充质干细胞（BM-MSCs）的分离培养方法，并比较不同年龄组BM-MSCs的生物学特性，为软骨缺损的自体干细胞治疗做准备。
选取接受脊柱手术的患者并按年龄分组。抽取患者的髂嵴骨髓。从骨髓中分离出BM-MSCs，然后进行培养。评估其生物学特性，包括形态外观和表面生物标志物。观察生长曲线。还进行了无菌和支原体检测以评估BM-MSCs培养过程的质量。
平均而言，培养的BM-MSCs在4至7周内达到7.56 - 22.95×10⁶数量。所有年龄组的BM-MSCs在形态、形状和表面生物标志物（CD105⁺、CD73⁺、CD34⁻、CD45⁻、CD14⁻、CD19⁻、HLA-DR⁻）方面表现出相同的质量。
我们分离培养BM-MSCs的方法已达到植入软骨损伤所需的数量。此外，培养的BM-MSCs的生物学特性也符合国际细胞治疗协会（ISCT）的MSCs标准。
