Biological and Environmental Sciences, School of Natural Sciences, University of Stirling, United Kingdom.
Am J Bot. 2011 Oct;98(10):e296-9. doi: 10.3732/ajb.1100159.
Microsatellite markers were developed using second-generation sequencing in Solanum rostratum as a tool to study the reproductive biology and genetic structure of this invasive species.
Thirteen microsatellites were successfully discovered and amplified in a single multiplexed PCR. All loci showed genetic variation in S. rostratum. Cross-amplification in five closely related taxa was successful for a subset of loci.
The set of 13 microsatellite markers developed here provides a time-effective and cost-effective genetic tool to study the reproductive biology of S. rostratum. The demonstrated transferability of the PCR multiplex to related taxa also highlights its usefulness for evolutionary studies across Solanum sect. Androceras.
本研究使用第二代测序技术在刺萼龙葵中开发了微卫星标记，以此作为研究该入侵物种生殖生物学和遗传结构的工具。
在单个多重 PCR 中成功发现并扩增了 13 个微卫星。所有位点在刺萼龙葵中均显示出遗传变异。一组亚位点在五个密切相关的分类群中成功进行了交叉扩增。
这里开发的 13 个微卫星标记集为研究刺萼龙葵的生殖生物学提供了一种省时、经济的遗传工具。PCR 多重扩增在相关分类群中的可转移性也突出了其在茄科龙葵族进化研究中的有用性。
