Center for Brain Repair and Rehabilitation, Institute for Neuroscience and Physiology, University of Gothenburg, and Sahlgrenska University Hospital, Box 432, S-40530 Gothenburg, Sweden.
Stroke. 2011 Dec;42(12):3559-65. doi: 10.1161/STROKEAHA.111.627802. Epub 2011 Oct 6.
Cortical ischemia induces neural progenitor cell migration toward the injury site; however, whether these cells are capable of maintaining the migratory response for a longer period after injury remains uncertain.
We analyzed progenitor migration up to 1 year after induction of photothrombotic stroke to the mouse neocortex. Migrating progenitors identified as doublecortin positive cells (DCX+) were assessed using the immunohistochemistry and immunofluorescence. The thymidine analogues chlorodeoxyuridine and iododeoxyuridine were used to birth-date the progenitor cells.
In the striatum, we detected elevated numbers of DCX+ cells up to 6 weeks postlesion. In the corpus callosum and the peri-infarct cortex (Ctx), DCX+ cell numbers were increased up to 1 year. The orientation of the migrating progenitors was mostly aligned with the corpus callosum fiber tract at all time points; however, in the Ctx, they aligned parallel to the infarct border. The injured cortex continuously receives new progenitors up to 1 year after lesion. Cells born after lesion did not become mature neurons, although a portion of the migrating progenitors showed initial signs of differentiation into neurons.
Neural progenitors might have a role in brain plasticity after cortical stroke, especially considering the prolonged window of migratory responses of up to 1 year after stroke lesion.
皮质缺血诱导神经祖细胞向损伤部位迁移；然而，这些细胞在损伤后是否能够维持更长时间的迁移反应尚不确定。
我们分析了光血栓性中风诱导后长达 1 年的祖细胞迁移。使用免疫组织化学和免疫荧光法评估作为双皮质素阳性细胞 (DCX+) 的迁移祖细胞。使用胸苷类似物氯脱氧尿苷和碘脱氧尿苷对祖细胞进行出生日期分析。
在纹状体中，我们检测到损伤后 6 周内 DCX+细胞数量增加。在胼胝体和梗死周围皮质 (Ctx) 中，DCX+细胞数量增加至 1 年。迁移祖细胞的方向在所有时间点都与胼胝体纤维束基本一致；然而，在 Ctx 中，它们与梗死边界平行排列。受伤的皮质在损伤后 1 年内不断接收新的祖细胞。出生于损伤后的细胞不会成为成熟神经元，尽管部分迁移祖细胞显示出向神经元分化的初步迹象。
神经祖细胞在皮质中风后的大脑可塑性中可能发挥作用，特别是考虑到中风损伤后长达 1 年的迁移反应的延长窗口。
