Department of Clinical Care Medicine, Division of Pharmacology and ESR Laboratories, Kanagawa Dental College, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan.
J Clin Biochem Nutr. 2011 Sep;49(2):79-86. doi: 10.3164/jcbn.10-103. Epub 2011 Jun 3.
Flavangenol, one of extract of French maritime pine bark, is a complex mixture of bioflavonoids with oligometric proanthocyanidins as the major constituents. These constituents, catechin and procyanidin B(1), are water-soluble derivatives of flavangenol. In this study, we investigated the antioxidant effects of flavangenol on reactive oxygen species such as hydroxyl radical, superoxide anion and singlet oxygen using electron spin resonance and spin trapping. The effect of flavangenol on oxidative stress in the skin from the maxillofacial region of hairless mice was investigated using an in vivo L-band electron spin resonance imaging system. Flavangenol attenuated oxidative stress in the maxillofacial skin by acting as a reactive oxygen species scavenger, as demonstrated by in vitro and in vivo electron spin resonance imaging analysis. The absorption and metabolism of flavangenol were also examined. After oral administration of flavangenol in human and rat, most of the catechin in plasma was in the conjugated form, while 45% to 78% of procyanidin B(1) was unconjugated, indicating that non-conjugated procyanidin B(1) would be active in the circulation. The ability of flavangenol to reduce reactive oxygen species levels in the circulation of the maxillofacial region suggests that this extract may be beneficial for skin protection from exposure to ultraviolet irradiation.
法国滨海松皮提取物中的黄烷醇是一种生物类黄酮的复杂混合物，以低聚原花青素为主要成分。这些成分，儿茶素和原花青素 B1，是黄烷醇的水溶性衍生物。在这项研究中，我们使用电子自旋共振和自旋捕获技术研究了黄烷醇对羟自由基、超氧阴离子和单线态氧等活性氧的抗氧化作用。使用体内 L 波段电子自旋共振成像系统研究了黄烷醇对无毛小鼠颌面皮肤氧化应激的影响。黄烷醇通过作为活性氧清除剂来减轻颌面皮肤的氧化应激，这通过体外和体内电子自旋共振成像分析得到了证明。还检查了黄烷醇的吸收和代谢。在人类和大鼠口服黄烷醇后，血浆中的大部分儿茶素呈共轭形式，而原花青素 B1 的 45%至 78%呈非共轭形式，表明非共轭原花青素 B1 将在循环中具有活性。黄烷醇降低颌面区域循环中活性氧水平的能力表明，这种提取物可能有益于皮肤免受紫外线照射的保护。
