Washiya Kiyotada, Sato Tatsusuke, Miura Tomisato, Tone Kiyosi, Kojima Keiko, Watanabe Jun, Kijima Hiroshi
Division of Medical Life Sciences, Department of Pathologic Analysis, Hirosaki University Graduate School of Health Sciences, Hirosaki, Japan.
Anal Quant Cytol Histol. 2011 Jun;33(3):169-74.
To follow cases with atypical cells or suspicious cases on first examination by liquid-based cytology (LBC), comparing cases that became negative and those confirmed to be positive (urothelial carcinoma) with regard to the cell morphology.
Urine cytology was performed employing LBC in 323 samples. Of 75 suspicious cases on first examination, 5 and 8 cases were identified with (positive) and without (negative) cancer on reexamination, respectively. Cell morphology was investigated in the first suspicious preparations of these cases.
Fewer cells were present in the samples of negative cases, and atypia was generally weak. The nuclei were swollen and pale, and hyperchromatism was weak. In contrast, in positive cases, nuclear swelling, flattening, shrinkage, and pale staining were less marked compared with those in negative cases. The nuclei were hyperchromatic and irregular in many cases. Nuclear cannibalism, multinucleation, anisokaryosis, three-dimensionality, and an irregular arrangement were considered to be features strongly suggesting malignancy.
The efficiency of diagnosis employing the LBC is high because of the cell collection rate. It was shown that the accuracy of diagnoses made employing the LBC method can be increased by understanding the characteristics of the cell morphology in suspicious cases.
对首次液基细胞学检查（LBC）发现非典型细胞的病例或可疑病例进行随访，比较复查后转阴病例和确诊为阳性（尿路上皮癌）病例的细胞形态。
采用LBC对323份样本进行尿液细胞学检查。首次检查的75例可疑病例中，复查时分别有5例和8例确诊为癌症（阳性）和未患癌症（阴性）。对这些病例首次可疑涂片的细胞形态进行研究。
阴性病例样本中的细胞较少，异型性通常较弱。细胞核肿胀且淡染，核深染较弱。相比之下，阳性病例中，与阴性病例相比，核肿胀、扁平、收缩及淡染不那么明显。许多病例的细胞核深染且不规则。核内假包涵体、多核、核大小不等、立体感及不规则排列被认为是强烈提示恶性肿瘤的特征。
由于细胞采集率，LBC诊断效率较高。结果表明，通过了解可疑病例细胞形态特征可提高LBC方法诊断的准确性。
