使用 iQPR-H₂O 进行骨再生及其在改善骨质疏松症方面的潜力。

Use of iQPR-H₂O for bone regeneration and its potential in the improvement of osteoporosis.

机构信息

Department of Oral Medicine, Taipei Medical University, Taipei, Taiwan.

出版信息

BMC Musculoskelet Disord. 2011 Oct 8;12:227. doi: 10.1186/1471-2474-12-227.

DOI:10.1186/1471-2474-12-227

PMID:21981964

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3206489/

Abstract

BACKGROUND

Current treatments for osteoporosis are associated with various side effects and do not prevent the age-related decrease in osteoblast number. The objective of this study was to evaluate the effects of iQPR-H₂O on osteogenesis.

METHODS

Mouse fibroblast NIH3T3 and pre-osteoblastic MC3T3-E1 cells were cultured in medium prepared with iQPR-H₂O or unprocessed mineral water (control cells), and proliferation and differentiation were assessed by MTT and alkaline phosphatase assay, respectively. Mineral deposition by the cells was determined using Alizarin red S staining. A mouse model of osteoporosis, ovariectomized SAMP8 mice, was used to evaluate the effects of iQPR-H₂O on osteogenesis in vivo. Mice were given either iQPR-H₂O or unprocessed mineral water (control group) for four months after which bone mass density (BMD) measurements were made using a bone densitometer and hematoxylin and eosin staining of bone samples.

RESULTS

NIH3T3 cells grown in medium prepared with iQPR-H₂O exhibited significantly greater proliferation. NIH3T3 and MC3T3-E1 cells demonstrated a significant increase in alkaline phosphatase levels in the iQPR-H₂O group. MC3T3-E1 cells showed mineralization at day 28. mRNA expression levels of both osteopontin and runt-related transcription factor 2 in MC3T3-E1 cells were higher in the iQPR-H₂O group compared with the control group. After four months, significantly greater bone regeneration was evident in ovariectomized SAMP8 mice administered iQPR-H₂O as compared with control group.

CONCLUSIONS

iQPR-H₂O may reduce the symptoms of osteoporosis by improving osteogenesis.

摘要

背景

目前骨质疏松症的治疗方法存在多种副作用，且不能预防与年龄相关的成骨细胞数量减少。本研究旨在评估 iQPR-H₂O 对成骨的影响。

方法

将小鼠成纤维细胞 NIH3T3 和前成骨细胞 MC3T3-E1 细胞分别在 iQPR-H₂O 或未处理矿泉水（对照细胞）培养基中培养，通过 MTT 和碱性磷酸酶测定分别评估增殖和分化，用茜素红 S 染色法测定细胞的矿化沉积。使用去卵巢 SAMP8 骨质疏松症小鼠模型评估 iQPR-H₂O 对体内成骨的影响。在去卵巢 4 个月后，用骨密度仪测量小鼠的骨密度（BMD），并对骨样本进行苏木精和伊红染色。

结果

在 iQPR-H₂O 培养基中培养的 NIH3T3 细胞表现出显著的增殖。NIH3T3 和 MC3T3-E1 细胞的碱性磷酸酶水平在 iQPR-H₂O 组中显著增加。MC3T3-E1 细胞在第 28 天出现矿化。与对照组相比，MC3T3-E1 细胞中骨桥蛋白和 runt 相关转录因子 2 的 mRNA 表达水平在 iQPR-H₂O 组中更高。4 个月后，与对照组相比，接受 iQPR-H₂O 治疗的去卵巢 SAMP8 小鼠的骨再生明显增加。