长期阴道扩张和β-氨基丙腈对尿控和尿道结构的影响。
Effects of prolonged vaginal distension and β-aminopropionitrile on urinary continence and urethral structure.
机构信息
Department of Urology, University of California, School of Medicine, San Francisco, CA, USA.
出版信息
Urology. 2011 Oct;78(4):968.e13-9. doi: 10.1016/j.urology.2011.07.1381.
OBJECTIVE
To investigate the effects of prolonged vaginal distension and β-aminopropionitrile (BAPN) on the urinary patterns and urethral structure in female virgin rats.
METHODS
A total of 21 female virgin rats were randomly divided into 3 groups of 7 rats each. The control group received no intervention; the vaginal distension (VD) group was treated with prolonged VD by balloon inflation; and the VD plus BAPN group was treated with VD plus intraperitoneal injection of 150 mg/kg of BAPN every 3 days. Three weeks later, all the rats underwent conscious cystometric analysis and were then killed for histologic analysis of the urethra.
RESULTS
Conscious cystometry identified 0, 3, and 5 rats in the control, VD, and VD plus BAPN groups with an abnormal voiding pattern, respectively. The urethral collagen content was significantly lower in the VD and VD plus BAPN rats compared with the control rats. The urethral elastic fibers were disorganized and shorter in the VD and VD plus BAPN rats and were fragmented and lacking the intermuscle connections in the VD plus BAPN rats. The urethral striated muscle fibers were shorter and more widely spaced in the VD and VD plus BAPN rats than in the control rats. Additionally, those in the VD plus BAPN group exhibited an abnormal wavy shape suggestive of a lack of architectural support.
CONCLUSION
Prolonged vaginal distension caused urodynamic changes and histologic abnormalities in the urethra, including reduced collagen content, fragmented elastic fibers, and sparsely arranged and shortened striated muscle fibers. BAPN appears to interfere with the restoration of collagen and elastic fibers.
目的
研究阴道扩张和β-氨基丙腈（BAPN）对雌性处女大鼠排尿模式和尿道结构的影响。
方法
将 21 只雌性处女大鼠随机分为 3 组，每组 7 只。对照组不进行干预；阴道扩张（VD）组通过球囊充气进行长时间 VD 治疗；VD 加 BAPN 组每隔 3 天用 150mg/kg 的 BAPN 进行腹腔注射。3 周后，所有大鼠进行清醒膀胱测压分析，然后处死进行尿道组织学分析。
结果
清醒膀胱测压分别在对照组、VD 组和 VD 加 BAPN 组中识别出 0、3 和 5 只具有异常排尿模式的大鼠。与对照组相比，VD 和 VD 加 BAPN 大鼠的尿道胶原含量明显降低。VD 和 VD 加 BAPN 大鼠的尿道弹性纤维排列紊乱且较短，在 VD 加 BAPN 大鼠中，弹性纤维断裂且缺乏肌间连接。VD 和 VD 加 BAPN 大鼠的尿道横纹肌纤维较对照组更短且间距更大。此外，VD 加 BAPN 组的肌纤维呈现异常波浪状，提示缺乏结构支撑。
结论
长时间阴道扩张导致尿道出现动力变化和组织学异常，包括胶原含量减少、弹性纤维断裂以及排列稀疏和缩短的横纹肌纤维。BAPN 似乎干扰了胶原和弹性纤维的恢复。
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