自动化 4D 分析树突棘形态：在疾病模型中应用于刺激诱导的树突棘重塑和药物干预。
Automated 4D analysis of dendritic spine morphology: applications to stimulus-induced spine remodeling and pharmacological rescue in a disease model.
机构信息
Department of Cell Biology, Emory University, 615 Michael St, NE, Atlanta, GA 30322, USA.
出版信息
Mol Brain. 2011 Oct 7;4:38. doi: 10.1186/1756-6606-4-38.
Abstract
Uncovering the mechanisms that regulate dendritic spine morphology has been limited, in part, by the lack of efficient and unbiased methods for analyzing spines. Here, we describe an automated 3D spine morphometry method and its application to spine remodeling in live neurons and spine abnormalities in a disease model. We anticipate that this approach will advance studies of synapse structure and function in brain development, plasticity, and disease.
摘要
揭示调节树突棘形态的机制在某种程度上受到缺乏有效和无偏的分析方法的限制。在这里，我们描述了一种自动的 3D 棘突形态测量方法及其在活神经元中的棘突重塑和疾病模型中的棘突异常中的应用。我们预计，这种方法将推进大脑发育、可塑性和疾病中突触结构和功能的研究。
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