Department of Radiology, School of Medicine, Patras University Hospital, Rion, Greece.
Cryobiology. 2011 Dec;63(3):267-72. doi: 10.1016/j.cryobiol.2011.09.138. Epub 2011 Sep 29.
To in vivo investigate the histological response after single and double cryoplasty therapy in a rabbit iliac artery model.
In total, 40 New Zealand White rabbits underwent percutaneous transluminal angioplasty of the iliac artery using either PolarCath balloon or a conventional angioplasty balloon of equal diameter. Arterial injury, inflammatory response and smooth muscle cells (SMC) apoptosis with the TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick End Labeling) immunohistochemical assay were analyzed. Rabbits were divided between single or double balloon inflation and histological results were compared between cryoplasty and control angioplasty both at 30 min and 72 h.
Arterial injury and wall inflammation scores were low and similar between cryoplasty and control groups after single and double balloon inflation. Compared to conventional balloon angioplasty, Polarcath cryoplasty demonstrated superior SMC apoptosis after single inflation at 30 min [12.0±1.2 cells/(0.025 mm)2 vs 7.0±1.5 cells/(0.025 mm)(2), p=0.002], single inflation at 72 h [9.0±1.0 cells/(0.025 mm)(2) vs 5.4±1.4 cells/(0.025 mm)(2), p=0.001], double inflation at 30 min [11.6±1.5 cells/(0.025 mm)(2) vs 6.8±1.4 cells/(0.025 mm)(2), p=0.001] and double inflation at 72h [9.2±0.8 cells/(0.025 mm)(2) vs 5.0±0.7 cells/(0.025 mm)(2), p=0.001]. There were no significant differences in apoptosis between single and double cryoplasty application at 30 min and 72 h.
Cryoplasty demonstrated superior rates of SMC apoptosis at 30 min and 72 h and was associated to relatively low arterial injury and inflammation scores. An immediate second PolarCath inflation did not achieve superior apoptosis.
在兔髂动脉模型中，体内研究单次和双次冷冻成形术治疗后的组织学反应。
共有 40 只新西兰白兔接受了经皮腔内血管成形术，使用的是 PolarCath 球囊或同等直径的常规血管成形术球囊。分析了动脉损伤、炎症反应和 TUNEL（末端脱氧核苷酸转移酶 dUTP 缺口末端标记）免疫组织化学检测的平滑肌细胞（SMC）凋亡。将兔子分为单次或双次球囊充气组，比较冷冻成形术和对照组在 30 分钟和 72 小时的组织学结果。
在单次和双次球囊充气后，冷冻成形术和对照组的动脉损伤和壁炎症评分均较低且相似。与常规球囊血管成形术相比，PolarCath 冷冻成形术在单次充气 30 分钟时显示出更好的 SMC 凋亡[12.0±1.2 个细胞/（0.025mm）2 比 7.0±1.5 个细胞/（0.025mm）2，p=0.002]，单次充气 72 小时时[9.0±1.0 个细胞/（0.025mm）2 比 5.4±1.4 个细胞/（0.025mm）2，p=0.001]，双次充气 30 分钟时[11.6±1.5 个细胞/（0.025mm）2 比 6.8±1.4 个细胞/（0.025mm）2，p=0.001]和双次充气 72 小时时[9.2±0.8 个细胞/（0.025mm）2 比 5.0±0.7 个细胞/（0.025mm）2，p=0.001]。在 30 分钟和 72 小时时，单次和双次冷冻成形术应用之间的凋亡没有显著差异。
冷冻成形术在 30 分钟和 72 小时时显示出更高的 SMC 凋亡率，与相对较低的动脉损伤和炎症评分相关。立即进行第二次 PolarCath 充气并不能获得更好的凋亡。
