Wu Zhengzhong, Zang Shengbing, Liu Wenwen, Jiang Na, Yang Weizhu
Department of Interventional Radiology, Fujian Medical University Union Hospital, Fuzhou, China.
Department of Pathology and Institute of Oncology, Fujian Medical University, Fuzhou, China.
Vasc Endovascular Surg. 2015 Jul-Aug;49(5-6):135-41. doi: 10.1177/1538574415603488. Epub 2015 Sep 2.
This study was performed to observe the effects of cryoplasty on canine iliac artery stenosis and the expression of tissue inhibition of matrix metalloproteinase 2 (TIMP-2) and matrix metalloproteinase 2 (MMP-2).
We produced a reliable canine model to mimic the atherosclerotic stenosis in the iliac artery by suturing the artery followed by vessel ligation to create an injury to intimal and medial walls. Sixteen mongrel dogs with iliac artery stenosis were randomized to conventional balloon angioplasty (n = 8) or cryoplasty (n = 8).
Four weeks posttreatment, the cryoplasty group with less collagen fibers and smooth muscle demonstrated significantly larger luminal diameter of iliac artery compared to the balloon angioplasty group (P < .001). Expression of TIMP-2 significantly increased and expression of MMP-2 significantly reduced in iliac artery of the cryoplasty group compared to conventional balloon angioplasty.
Our study suggests cryoplasty might increase the expression of TIMP-2 and decrease the expression of MMP-2, thereby inhibiting vascular hyperplasia and collagen fibers synthesis of the stenotic vessels.
本研究旨在观察冷冻球囊血管成形术对犬髂动脉狭窄的影响以及基质金属蛋白酶2组织抑制因子（TIMP-2）和基质金属蛋白酶2（MMP-2）的表达情况。
我们通过缝合动脉并进行血管结扎以造成内膜和中膜损伤，制作了一个可靠的模拟犬髂动脉粥样硬化狭窄的模型。16只患有髂动脉狭窄的杂种犬被随机分为传统球囊血管成形术组（n = 8）和冷冻球囊血管成形术组（n = 8）。
治疗后4周，与球囊血管成形术组相比，冷冻球囊血管成形术组胶原纤维和平滑肌较少，髂动脉管腔直径明显更大（P <.001）。与传统球囊血管成形术相比，冷冻球囊血管成形术组髂动脉中TIMP-2的表达显著增加，MMP-2的表达显著降低。
我们的研究表明，冷冻球囊血管成形术可能会增加TIMP-2的表达并降低MMP-2的表达，从而抑制狭窄血管的血管增生和胶原纤维合成。
