Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Faculty of science, University of Utrecht, Universiteitsweg 99, 3584 CG Utrecht, The Netherlands.
J Control Release. 2012 Mar 28;158(3):433-42. doi: 10.1016/j.jconrel.2011.09.078. Epub 2011 Sep 24.
High-content screening (HCS) uses high-capacity automated fluorescence imaging for the quantitative analysis of single cells and cell populations. Here, we developed an HCS assay for rapid screening of non-viral gene delivery systems as exemplified by the screening of a small library of peptide-based transfectants. These peptides were simultaneously screened for transfection efficiency, cytotoxicity, induction of cell permeability and the capacity to transfect non-dividing cells. We demonstrated that HCS is a valuable extension to the already existing screening methods for the in vitro evaluation of non-viral gene delivery systems with the added value that multiple parameters can be screened in parallel thereby obtaining more information from a single screening event, which will accelerate the development of novel gene delivery systems.
高内涵筛选（HCS）使用大容量自动化荧光成像技术对单细胞和细胞群体进行定量分析。在这里，我们开发了一种 HCS 测定法，用于快速筛选非病毒基因传递系统，例如筛选一小批基于肽的转染体。同时筛选这些肽的转染效率、细胞毒性、诱导细胞通透性以及转染非分裂细胞的能力。我们证明 HCS 是体外评估非病毒基因传递系统的现有筛选方法的一个有价值的扩展，其附加价值在于可以同时筛选多个参数，从而从单个筛选事件中获得更多信息，这将加速新型基因传递系统的开发。
