Department of Psychological and Brain Sciences, Neurogenetics and Behavior Center, Johns Hopkins University, 3400 N. Charles Street, Ames Hall, Room 101, Baltimore, MD 21218, USA.
Appetite. 2012 Feb;58(1):215-21. doi: 10.1016/j.appet.2011.09.015. Epub 2011 Sep 29.
The current studies examined the influence of a high fat diet on sucrose acceptance in diet induced obese (DIO) mice. C57BL/6J mice were placed on either a 45 kcal% fat diet (group DIO), or a control 10% kcal fat diet (group control) for 12 weeks followed by sucrose consumption tests and dietary manipulations. After 12 weeks exposure, body weights of DIO mice significantly exceeded those of the control mice. During subsequent sucrose consumption tests, DIO mice showed suppression in the total number of licks relative to controls. In a second experiment, consumption tests with water and a variety of sucrose concentrations revealed a hypophagic phenotype in naïve DIO mice. Licking microstructure analyses were conducted on the licking behavior of all mice, which revealed a reduction in burst size and number for DIO mice. Subsequently, we examined whether 10 days exposure to regular lab chow would alter sucrose consumption and taste evaluation in DIO mice. As a result of this dietary switch, all mice showed comparable licking behavior suggesting that exposure to the high-fat diet and diet-induced obesity may reduce preferences for other tastants in C57BL/6J mice.
目前的研究考察了高脂肪饮食对饮食诱导肥胖（DIO）小鼠蔗糖接受能力的影响。将 C57BL/6J 小鼠分别置于 45%卡路里脂肪饮食（DIO 组）或 10%卡路里脂肪对照饮食（对照组）中 12 周，然后进行蔗糖消耗测试和饮食操作。经过 12 周的暴露，DIO 小鼠的体重明显超过了对照组。在随后的蔗糖消耗测试中，DIO 小鼠的总舔舐次数相对对照组减少。在第二项实验中，对水和各种蔗糖浓度的消耗测试显示，DIO 小鼠在初始状态下表现出食欲减退的表型。对所有小鼠的舔舐行为进行微观结构分析，结果显示 DIO 小鼠的爆发大小和数量减少。随后，我们研究了 10 天暴露于常规实验室饲料是否会改变 DIO 小鼠的蔗糖消耗和味觉评估。由于这种饮食转换，所有小鼠的舔舐行为相似，表明暴露于高脂肪饮食和饮食诱导的肥胖可能会降低 C57BL/6J 小鼠对其他味觉剂的偏好。
