Department of Biological Sciences, Evolutionary and Comparative Biology Division, University of Naples Federico II, Naples, Italy.
Gen Comp Endocrinol. 2011 Dec 1;174(3):318-25. doi: 10.1016/j.ygcen.2011.09.009. Epub 2011 Sep 29.
In vertebrates, the liver was long thought to be the only site of vitellogenin (Vtg) production, but recent studies demonstrated that Vtg is also expressed in extrahepatic districts. The aim of this paper is to assess, by in situ hybridization and immunohistochemistry, the expression of Vtg in the testis and kidney of Torpedo marmorata exposed to 17β-estradiol (E(2)). In treated samples vtg mRNA and Vtg were detected contemporaneously only in the testis; differently the kidney cells were positive to Vtg antibody, but negative to vtg mRNA. This is the first study to assess that male germ cells, after an exposure to E(2), synthesize Vtg in a stage-dependent manner. The presence of Vtg and the modifications observed in the kidney after E(2) treatment are discussed.
在脊椎动物中，肝脏长期以来被认为是卵黄蛋白原（Vtg）产生的唯一部位，但最近的研究表明，Vtg 也在肝外区域表达。本文的目的是通过原位杂交和免疫组织化学评估暴露于 17β-雌二醇（E（2））的蓝斑马鲛鱼睾丸和肾脏中 Vtg 的表达。在处理过的样本中，仅在睾丸中同时检测到 vtg mRNA 和 Vtg；而在肾脏细胞中，Vtg 抗体呈阳性，但 vtg mRNA 呈阴性。这是第一项评估雄性生殖细胞在暴露于 E（2）后以依赖于阶段的方式合成 Vtg 的研究。讨论了 Vtg 的存在以及 E（2）处理后肾脏中观察到的变化。
