Department of Biological Sciences, Evolutionary and Comparative Biology Division, University of Naples Federico II, Naples, Italy.
J Fish Biol. 2012 Apr;80(5):2112-21. doi: 10.1111/j.1095-8649.2011.03172.x. Epub 2012 Jan 5.
The aim of this investigation was to assess the effects of nonylphenol (NP), an oestrogen-like environmental pollutant, on the vitellogenin (VTG) synthesis in adult males of the aplacental viviparous cartilaginous fish Torpedo marmorata. The VTG recovery in males is considered a biomarker of xeno-oestrogenic pollution as this lipophosphoglycoprotein is physiologically induced by oestrogens only in females of oviparous and ovoviparous vertebrates. Using in situ hybridization and immunohistochemistry, T. marmorata males injected with nonylphenol showed the presence of VTG in the liver and the kidney. In particular, vtg messenger (m)RNA and VTG protein were expressed in the liver, whereas in the kidney cells only the presence of VTG was recorded. By contrast, no expression for VTG was detected in the testis. These results demonstrate that in T. marmorata NP induces the expression of vtg only in the liver; the presence of VTG in the kidney and its absence in the testis are discussed.
本研究旨在评估壬基酚（NP），一种类雌激素环境污染物，对有胎盘的软骨鱼类尖吻鲛（Torpedo marmorata）成年雄性个体卵黄蛋白原（VTG）合成的影响。雄性个体的 VTG 恢复被认为是一种外源性雌激素污染的生物标志物，因为这种脂蛋白在卵生和卵胎生脊椎动物的雌性中仅受雌激素生理诱导。通过原位杂交和免疫组织化学方法，注射壬基酚的尖吻鲛雄性个体在肝脏和肾脏中均检测到 VTG 的存在。具体而言，在肝脏中表达了 vtg 信使（m）RNA 和 VTG 蛋白，而在肾脏细胞中仅记录到 VTG 的存在。相比之下，在睾丸中未检测到 VTG 的表达。这些结果表明，在尖吻鲛中，NP 仅在肝脏中诱导 vtg 的表达；在肾脏中存在 VTG 而在睾丸中不存在 VTG 的情况被讨论。
