School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Food Chem Toxicol. 2011 Dec;49(12):3395-406. doi: 10.1016/j.fct.2011.08.013. Epub 2011 Sep 29.
Our study goals to investigate the anti-arthritic potential of Salix nigra bark methanol extract (SNME) against both inflammation and oxidative stress in the collagen-induced arthritis (CIA) rat model. Results showed that SNME exhibited maximum scavenging activity against superoxide, hypochlorous acid and hydrogen peroxide radicals along with the suppression of lipid peroxidation. Female wistar rats were immunized with porcine type II collagen and treated with SNME (100 mg/kg body weight) for 15 days starting on day 20. SNME significantly inhibited the paw swelling and arthritic score; exhibited maximum CIA inhibition of 93.7% by the end of the experimental period. Administration of SNME to arthritic rats significantly improved the histological findings in joints as evident by reduced infiltration of polymorphonuclear cells and smooth synovial lining. Roentgenograms of tibiotarsal joints of both SNME and indomethacin-treated rats showed protection against osteophyte formation, soft tissue swelling and bone resorption. Furthermore, levels of inflammatory mediators (nitric oxide, TNF-α, IL-1β, IL-6) measured in both plasma and joint exudates were significantly reduced by SNME treatment. Increased oxidative stress observed in the arthritic animals was also found to be significantly restored in SNME- treated rats. Taken together, our studies clearly indicate the potential of S. nigra as an anti-arthritic agent.
我们的研究旨在探讨黑柳皮甲醇提取物（SNME）对胶原诱导性关节炎（CIA）大鼠模型中炎症和氧化应激的抗关节炎潜力。结果表明，SNME 对超氧阴离子、次氯酸和过氧化氢自由基表现出最大的清除活性，并抑制脂质过氧化。雌性 wistar 大鼠用猪 II 型胶原免疫，并从第 20 天开始用 SNME（100mg/kg 体重）治疗 15 天。SNME 显著抑制足肿胀和关节炎评分；在实验结束时，对 CIA 的抑制作用最大，达到 93.7%。给予 SNME 治疗关节炎大鼠可显著改善关节的组织学发现，减少多形核细胞浸润和滑膜衬里的平滑。SNME 和吲哚美辛治疗大鼠的胫跗关节射线照片显示可防止骨赘形成、软组织肿胀和骨吸收。此外，SNME 治疗还显著降低了血浆和关节渗出物中炎症介质（一氧化氮、TNF-α、IL-1β、IL-6）的水平。在关节炎动物中观察到的氧化应激增加也被发现可在 SNME 治疗大鼠中得到显著恢复。综上所述，我们的研究清楚地表明黑柳具有作为抗关节炎药物的潜力。
