Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, Brazil.
Fish Physiol Biochem. 2012 Jun;38(3):797-805. doi: 10.1007/s10695-011-9563-3. Epub 2011 Oct 8.
Aquatic organisms are continuously exposed to environmental variations, which can lead to physiological and biochemical alterations. Leporinus macrocephalus, known as piavuçu, is a migratory species that may be exposed to variations in dissolved oxygen levels. Studies evaluating oxidative changes undergone by this species in these conditions are scarce. Therefore, this investigation aimed at evaluating oxidative alterations in L. macrocephalus exposed to different oxygen levels for 96 h: 6.12 ± 0.18, 3.99 ± 0.17, 3.22 ± 0.17, 2.47 ± 0.30 and 0.710 ± 0.07 mg L(-1). At the end of the experimental period, fish were euthanized and livers used to determine lipid hydroperoxides, thiobarbituric acid reactive substances, catalase, glutathione-S-transferase, superoxide dismutase and thiol groups, which are an indirect measure of reduced glutathione. Results indicated a decrease in the studied parameters in hypoxic situations, suggesting a possible metabolic depression.
水生生物不断暴露于环境变化之中,这可能导致其生理和生化发生改变。脂鲤属的大头鱼,又名皮亚乌库,是一种洄游鱼类,可能会接触到溶解氧水平的变化。评估该物种在这些条件下发生的氧化变化的研究很少。因此,本研究旨在评估大头鱼在暴露于不同氧水平 96 小时后的氧化变化:6.12 ± 0.18、3.99 ± 0.17、3.22 ± 0.17、2.47 ± 0.30 和 0.710 ± 0.07 mg L(-1)。在实验期末,对鱼类进行安乐死,并使用肝脏来测定脂质过氧化物、硫代巴比妥酸反应物质、过氧化氢酶、谷胱甘肽-S-转移酶、超氧化物歧化酶和巯基,这些是还原型谷胱甘肽的间接测量指标。结果表明,在缺氧情况下,研究参数下降,这表明可能存在代谢抑制。