Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Science City, Japan.
EMBO Mol Med. 2011 Dec;3(12):742-54. doi: 10.1002/emmm.201100177. Epub 2011 Oct 10.
In the present study, we demonstrated that insulin is produced not only in the mammalian pancreas but also in adult neuronal cells derived from the hippocampus and olfactory bulb (OB). Paracrine Wnt3 plays an essential role in promoting the active expression of insulin in both hippocampal and OB-derived neural stem cells. Our analysis indicated that the balance between Wnt3, which triggers the expression of insulin via NeuroD1, and IGFBP-4, which inhibits the original Wnt3 action, is regulated depending on diabetic (DB) status. We also show that adult neural progenitors derived from DB animals retain the ability to give rise to insulin-producing cells and that grafting neuronal progenitors into the pancreas of DB animals reduces glucose levels. This study provides an example of a simple and direct use of adult stem cells from one organ to another, without introducing additional inductive genes.
在本研究中，我们证明胰岛素不仅在哺乳动物胰腺中产生，而且在海马体和嗅球（OB）衍生的成年神经元细胞中产生。旁分泌 Wnt3 在促进海马体和 OB 衍生的神经干细胞中胰岛素的活性表达中发挥重要作用。我们的分析表明，触发胰岛素通过 NeuroD1 表达的 Wnt3 与抑制原始 Wnt3 作用的 IGFBP-4 之间的平衡取决于糖尿病（DB）状态。我们还表明，源自 DB 动物的成年神经祖细胞保留产生胰岛素产生细胞的能力，并且将神经元祖细胞移植到 DB 动物的胰腺中会降低葡萄糖水平。本研究提供了一个简单而直接地使用来自一个器官的成年干细胞到另一个器官的例子，而无需引入额外的诱导基因。
