Nazir Mamona, Sultan Misbah, Riaz Naheed, Hafeez Maria, Hussain Hidayat, Ahmed Ishtiaq, Schulz Barbara, Draeger Siegfried, Jabbar Abdul, Krohn Karsten, Ashraf Muhammad, Saleem Muhammad
Department of Chemistry , Baghdad-ul-Jadeed Campus, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan.
J Asian Nat Prod Res. 2011 Oct;13(11):1056-60. doi: 10.1080/10286020.2011.606811. Epub 2011 Oct 10.
Chromatographic purification of the extract of an endophytic fungal culture yielded depsitinuside (1), a new phenolic ester together with ergosterol (2) and (22E,24S)-24-methyl-5-α-cholesta-7,22-diene-3β,5,6β-triol (3). The structure of 1 was elucidated based on 1D, 2D NMR spectroscopy and high-resolution mass spectrometry, whereas the known compounds (2 and 3) were identified by (1)H NMR, mass spectrometry, and in comparison with the literature values. Compound 1 was evaluated for its enzyme inhibitory potential against acetylcholinesterase, butyrylcholinesterase and lipoxygenase, and was found inactive (10%-40% inhibition at a concentration of 2 mg/ml).
对一种内生真菌培养物提取物进行色谱纯化，得到了depsitinuside（1），一种新的酚酯，以及麦角甾醇（2）和（22E，24S）-24-甲基-5-α-胆甾-7，22-二烯-3β，5，6β-三醇（3）。通过一维、二维核磁共振光谱和高分辨率质谱对1的结构进行了阐明，而已知化合物（2和3）则通过氢核磁共振、质谱并与文献值进行比较来鉴定。对化合物1针对乙酰胆碱酯酶、丁酰胆碱酯酶和脂氧合酶的酶抑制潜力进行了评估，发现其无活性（在浓度为2 mg/ml时抑制率为10%-40%）。
