光诱导 RNA 干扰的光敏载体蛋白。

Photosensitizing carrier proteins for photoinducible RNA interference.

机构信息

Department of Bioscience and Biotechnology, Okayama University , 3-1-1 Tsushimanaka, Okayama, Japan.

出版信息

Bioconjug Chem. 2011 Nov 16;22(11):2222-6. doi: 10.1021/bc200095a. Epub 2011 Oct 27.

DOI:10.1021/bc200095a

PMID:21985108

Abstract

RNA interference (RNAi) is being widely explored as a tool in functional genomics and tissue engineering, and in the therapy of intractable diseases, including cancer and neurodegenerative diseases. Recently, we developed a photoinducible RNAi method using photosensitizing carrier proteins, named CLIP-RNAi (CPP-linked RBP-mediated RNA internalization and photoinduced RNAi). Novel carrier proteins were designed for this study to establish a highly efficient delivery system for small interfering RNA (siRNA) or short hairpin RNA (shRNA) and to demonstrate light-dependent gene silencing. In addition, the results suggested that the dissociation of the siRNA (or shRNA) from carrier proteins in the cytoplasm is a critical event in CLIP-RNAi-mediated gene silencing.

摘要

RNA 干扰 (RNAi) 作为功能基因组学和组织工程学以及治疗难治性疾病（包括癌症和神经退行性疾病）的工具，正得到广泛的探索。最近，我们使用光敏载体蛋白开发了一种光诱导的 RNAi 方法，命名为 CLIP-RNAi（CPP 连接的 RBP 介导的 RNA 内化和光诱导的 RNAi）。为了建立高效的小干扰 RNA (siRNA) 或短发夹 RNA (shRNA) 传递系统，并证明光依赖性基因沉默，本研究设计了新型载体蛋白。此外，结果表明，siRNA（或 shRNA）从载体蛋白在细胞质中的解离是 CLIP-RNAi 介导的基因沉默的关键事件。