Federal University of Mato Grosso, Gynecology and Obstetrics, Cuiabá, Brazil.
Climacteric. 2011 Dec;14(6):677-82. doi: 10.3109/13697137.2011.570387. Epub 2011 Oct 10.
To evaluate the cellular and humoral immune responses after oral hormone therapy in postmenopausal women. Study design This was a prospective cohort study, with intervention. The main outcome measures were delayed-type IV cell-mediated hypersensitivity, leukocytes, immunoglobulins, interleukin-6 (IL-6) and interleukin-10 (IL-10).
The delayed-type cell-mediated hypersensitivity was measured by using five common allergens before and after 3 months of hormone therapy. Each type of leukocyte cell was counted before and after hormone therapy. Different subtypes of lymphocytes were determined by flow cytometry. Immunoglobulins G, A and M were measured by nephelometry; immunoglobulin E was measured by electrochemiluminescence. IL-6 and IL-10 concentrations were determined by chemiluminescence.
Hormone therapy increased the response to tuberculin antigen without changing the total number of leukocytes, eosinophils, neutrophils, lymphocytes, and CD4 +, CD8 + B cells. Both monocyte number and CD4 + /CD8 + ratio suffered a slight modification (p = 0.057). Immunoglobulins A, M and E remained unchanged and immunoglobulin G decreased (p = 0.029). IL-6 levels remained stable but IL-10 concentrations increased significantly after hormone therapy.
Short-term oral hormone treatment has no impact on the cellular immune response but, concerning the humoral immune response, immunoglobulin G decreased and the levels of IL-10 were significantly higher.
评估绝经后女性口服激素治疗后的细胞和体液免疫应答。
这是一项前瞻性队列研究，有干预措施。主要观察指标为迟发型 IV 型细胞介导的超敏反应、白细胞、免疫球蛋白、白细胞介素 6（IL-6）和白细胞介素 10（IL-10）。
在激素治疗前和治疗后 3 个月，使用五种常见过敏原测量迟发型细胞介导的超敏反应。在激素治疗前后分别计数白细胞的各细胞类型。通过流式细胞术确定淋巴细胞的不同亚型。用散射比浊法测量免疫球蛋白 G、A 和 M；用电化学发光法测量免疫球蛋白 E。用化学发光法测定 IL-6 和 IL-10 浓度。
激素治疗增加了对结核菌素抗原的反应，而不改变白细胞总数、嗜酸性粒细胞、中性粒细胞、淋巴细胞和 CD4+、CD8+B 细胞。单核细胞数量和 CD4+/CD8+比值略有变化（p=0.057）。免疫球蛋白 A、M 和 E 保持不变，免疫球蛋白 G 减少（p=0.029）。IL-6 水平保持稳定，但激素治疗后 IL-10 浓度显著增加。
短期口服激素治疗对细胞免疫应答没有影响，但对体液免疫应答而言，免疫球蛋白 G 减少，IL-10 水平显著升高。
