Itaborahy R M Ribeiro, de Medeiros S Freitas
a Federal University of Mato Grosso, School of Medicine , Department of Obstetrics and Gynecology , Cuiabá , Brazil.
Climacteric. 2016 Oct;19(5):496-500. doi: 10.1080/13697137.2016.1212828. Epub 2016 Sep 3.
To evaluate the impact of estrogen therapy on cellular and humoral immune markers in postmenopausal women.
This prospective, controlled cohort study included 30 patients who used oral estradiol (1 mg) for 14-17 weeks and 28 patients who served as controls. Total leukocytes and leukocyte subtypes were counted and immunophenotyped by flow cytometry. The concentrations of immunoglobulins and pro- and anti-inflammatory cytokines were also measured in the peripheral blood before and after estrogen therapy. Immunoglobulin E level was measured by electrochemiluminescence, and levels of immunoglobulins A, G, and M were measured by nephelometry. Simultaneous quantification of multiple cytokines was performed by chemiluminescence to measure the serum concentrations of interferon gamma, interleukin (IL)-4, IL-6, IL-10, and IL-17.
Hematological cellular components were not significantly different before and after the use of estradiol (p = 0.332-0.984). Serum concentrations of immunoglobulins G, M, E, and A also remained stable (p = 0.248-0.845). Finally, cytokines were not modified throughout the 14-17 weeks of follow-up (p = 0.407-0.873).
Isolated estrogen therapy with 1 mg of estradiol for 14-17 weeks in postmenopausal women did not modify any of the cellular or humoral immune markers analyzed in this study.
评估雌激素治疗对绝经后女性细胞免疫和体液免疫标志物的影响。
这项前瞻性对照队列研究纳入了30例口服雌二醇（1毫克）14 - 17周的患者和28例作为对照的患者。通过流式细胞术对全血细胞和白细胞亚型进行计数及免疫表型分析。在雌激素治疗前后还对外周血中的免疫球蛋白、促炎和抗炎细胞因子浓度进行了检测。免疫球蛋白E水平通过电化学发光法测定，免疫球蛋白A、G和M水平通过散射比浊法测定。通过化学发光法同时对多种细胞因子进行定量，以测量血清中干扰素γ、白细胞介素（IL）-4、IL-6、IL-10和IL-17的浓度。
使用雌二醇前后血液学细胞成分无显著差异（p = 0.332 - 0.984）。免疫球蛋白G、M、E和A的血清浓度也保持稳定（p = 0.248 - 0.845）。最后，在14 - 17周的随访期间细胞因子未发生改变（p = 0.407 - 0.873）。
绝经后女性单独使用1毫克雌二醇进行14 - 17周的雌激素治疗，并未改变本研究中分析的任何细胞免疫或体液免疫标志物。
