Advanced Institutes for Interdisciplinary Research, East China Normal University, 3663 North Zhongshan Road, Shanghai 200062, China.
Brain Res Bull. 2012 Jan 4;87(1):30-6. doi: 10.1016/j.brainresbull.2011.09.020. Epub 2011 Oct 1.
GPR12, an orphan G protein-coupled receptor, constitutively activates the Gs signaling pathway and further increases intracellular cyclic AMP. GPR12 overexpression has been reported to promote neurite extension in neurons or transform neuro2a neuroblastoma cells into neuron-like cells. However, the possible effects and mechanisms of GPR12 in the differentiation of PC12 cells are still unknown. The present study shows that GPR12 overexpression induced PC12 cells differentiation into neuron-like cells with enlarged cell sizes and neuritogenesis possibly via activation of Erk1/2 signaling and significantly increased the expression of several neurite outgrowth-related genes, including Bcl-xL, Bcl-2 and synaptophysin. These findings indicate that GPR12 may play a role in neurite outgrowth during PC12 cell differentiation.
GPR12 是一种孤儿 G 蛋白偶联受体，它会持续激活 Gs 信号通路，进一步增加细胞内的环腺苷酸。已有报道称，GPR12 的过表达可促进神经元中的轴突延伸，或将神经母细胞瘤细胞Neuro2a 转化为神经元样细胞。然而，GPR12 在 PC12 细胞分化中的可能作用和机制仍不清楚。本研究表明，GPR12 的过表达诱导 PC12 细胞分化为具有增大细胞体积和神经突生成的神经元样细胞，这可能是通过激活 Erk1/2 信号通路实现的，并且显著增加了几个与神经突生长相关的基因的表达，包括 Bcl-xL、Bcl-2 和突触素。这些发现表明，GPR12 可能在 PC12 细胞分化过程中的神经突生长中发挥作用。
