Brink Peter R, Valiunas Virginijus, Gordon Chris, Rosen Michael R, Cohen Ira S
Department of Physiology and Biophysics, Stony Brook University, Stony Brook, NY, USA.
Biochim Biophys Acta. 2012 Aug;1818(8):2076-81. doi: 10.1016/j.bbamem.2011.09.025. Epub 2011 Oct 2.
In vivo delivery of small interfering RNAs (siRNAs) to target cells via the extracellular space has been hampered by dilution effects and immune responses. Gap junction-mediated transfer between cells avoids the extracellular space and its associated limitations. Because of these advantages cell based delivery via gap junctions has emerged as a viable alternative for siRNA or miRNA delivery. Here we discuss the advantages and disadvantages of extracellular delivery and cell to cell delivery via gap junction channels composed of connexins. This article is part of a Special Issue entitled: The Communicating junctions, composition, structure and characteristics.
通过细胞外空间将小分子干扰RNA（siRNA）体内递送至靶细胞一直受到稀释效应和免疫反应的阻碍。细胞间通过间隙连接介导的转移可避免细胞外空间及其相关限制。由于这些优势，基于间隙连接的细胞递送已成为siRNA或miRNA递送的一种可行替代方法。在这里，我们讨论了通过连接蛋白组成的间隙连接通道进行细胞外递送和细胞间递送的优缺点。本文是名为：通讯连接、组成、结构和特性的特刊的一部分。
