非霍奇金淋巴瘤细针穿刺抽吸物的最佳标本处理

Optimal specimen processing of fine needle aspirates of non-Hodgkin lymphoma.

作者信息

Shetuni Brandon, Lakey Meredith, Kulesza Peter

机构信息

Department of Pathology, The Feinberg School of Medicine, Northwestern University, Chicago, Illinois.

出版信息

Diagn Cytopathol. 2012 Nov;40(11):984-6. doi: 10.1002/dc.21780. Epub 2011 Oct 11.

DOI:10.1002/dc.21780

PMID:21987470

Abstract

UNLABELLED

Fine needle aspiration (FNA) in conjunction with flow cytometry (FC) is a useful technique for non-Hodgkin's lymphoma (NHL) diagnosis. We sought to investigate the effect of storage medium and time to processing on lymph node (LN) FNA viability. Benign LN FNAs were distributed among Roswell Park Memorial Institute (RPMI), Hanks' Balanced Salt Solution (HBSS) and Dulbecco's Modified Eagle's Medium (DMEM) storage media, and viability was compared at 0, 4.5 and 24 hours. FC survival analysis showed viable cells (%): at 0 hrs: HBSS 83.6%, RPMI 87.7%, DMEM 87.7%. At 4.5 hrs: HBSS 86.3%, RPMI 89.0%, DMEM 78.2%. At 24 hrs: HBSS 82.7%, RPMI 86.7%, DMEM 77.2%. FNA from a peri-pancreatic LN involved by grade 2 follicular lymphoma was stored in RPMI at 4° C and analyzed at 1, 3, 5 and 7 days. Over 90% of follicular lymphoma cells were suitable for FC analysis at 1, 3, and 5 days after collection, decreasing to 76% at 7 days.

IN CONCLUSION

RPMI appears to be the optimal storage medium compared to DMEM and HBSS.An NHL FNA sample stored at 4° C remains suitable for FC analysis for up to 5 days.

摘要

未标记

细针穿刺抽吸（FNA）联合流式细胞术（FC）是诊断非霍奇金淋巴瘤（NHL）的一项有用技术。我们试图研究储存介质和处理时间对淋巴结（LN）FNA活力的影响。将良性LN FNA样本分别置于罗斯韦尔公园纪念研究所（RPMI）培养基、汉克斯平衡盐溶液（HBSS）和杜氏改良伊格尔培养基（DMEM）中，并在0、4.5和24小时时比较其活力。FC生存分析显示活细胞百分比：0小时时：HBSS为83.6%，RPMI为87.7%，DMEM为87.7%；4.5小时时：HBSS为86.3%，RPMI为89.0%，DMEM为78.2%；24小时时：HBSS为82.7%，RPMI为86.7%，DMEM为77.2%。取自2级滤泡性淋巴瘤累及的胰腺周围LN的FNA样本在4℃下保存在RPMI中，并在第1、3、5和7天进行分析。收集后1、3和5天，超过90%的滤泡性淋巴瘤细胞适合FC分析，到第7天时降至76%。