Sapia S, Sánchez Avalos J C, Monreal M, Galeano A, González Villaveiran R, Cornicelli J, Gamboni M
Sanatorio Mater Dei, Buenos Aires, Argentina.
Medicina (B Aires). 1995;55(6):675-80.
The aim was to evaluate the usefulness of lymph node biopsies obtained by fine needle aspiration (FNA) for immunophenotyping of non Hodgkin lymphoma (NHL). Seventeen superficial and deep lymph node samples were fractioned for conventional cytological examination and immunophenotyping studies. Out of ten NHL, nine were readily detected by flow cytometry (FC), while failure on the remaining case was due to selective loss of large cell population, which is liable to occur with this procedure. A single case, which proved negative for all markers employed, was finally diagnosed by immunohistochemistry as germ cell tumor. The other six cases, presenting lymphoid population without phenotypic abnormalities, were diagnosed by cytology and/or histology as Hodgkin disease or hyperplasic disorders. To conclude, FC immunophenotyping seems to improve the efficacy of FNA in NHL diagnosis, whereas for Hodgkin disease and hyperplasic disorders, classic morphological criteria are more useful for differential diagnosis. Although FNA for FC immunophenotyping cannot replace histopathological examination for NHL diagnosis, it proves to be a useful tool for staging and follow up, making surgical procedures for sample collection unnecessary.
目的是评估通过细针穿刺抽吸（FNA）获取的淋巴结活检对非霍奇金淋巴瘤（NHL）免疫表型分析的有用性。对17个浅表和深部淋巴结样本进行分割，用于常规细胞学检查和免疫表型分析研究。在10例NHL中，9例通过流式细胞术（FC）很容易检测到，而其余1例检测失败是由于大细胞群体的选择性丢失，这种情况在该操作中容易发生。1例对所有使用的标记物均呈阴性的病例，最终通过免疫组织化学诊断为生殖细胞肿瘤。另外6例表现为无表型异常的淋巴细胞群体的病例，通过细胞学和/或组织学诊断为霍奇金病或增生性疾病。总之，FC免疫表型分析似乎提高了FNA在NHL诊断中的效能，而对于霍奇金病和增生性疾病，经典的形态学标准对鉴别诊断更有用。虽然用于FC免疫表型分析的FNA不能替代NHL诊断的组织病理学检查，但它被证明是分期和随访的有用工具，无需进行手术采集样本。
