Department of Chemistry, School of Engineering, Brown University, Providence, Rhode Island 02912, USA.
J Biomed Mater Res A. 2012 Jan;100(1):94-102. doi: 10.1002/jbm.a.33217. Epub 2011 Oct 11.
Biomaterial nanotopographies have been proposed as a means to significantly influence cell functions (including osteoblasts, fibroblasts, endothelial cells, chondrocytes, immune cells, and bacteria). In this study, lung epithelial carcinoma cell functions including adhesion (up to 4 h), proliferation (up to 3 days), apoptosis (up to 5 days), and vascular endothelial growth factor (VEGF) synthesis (up to 5 days) on poly-lactic-co-glycolic (PLGA) films with various nanotopographies were systematically investigated. Importantly, this study created PLGA films with various nanotopographies (specifically, nano-smooth, 23, 300, and 400 nm hemispherical surface features) but similar surface chemistry in order to focus only on the effect of PLGA topography on cancer cell functions. Simple and effective cast-molding and solvent evaporation methods were used to accomplish this. Atomic force microscopy, electron spectroscopy for chemical analysis, and water contact angle measurements verified similar surface chemistry and energy but varied topographies for all of the PLGA films prepared in this study. Lung epithelial carcinoma cell adhesion, proliferation, and morphology results indicated less cell growth and spreading on nano-smooth and 400 nm surface-featured PLGA compared to any other samples. However, results also demonstrated decreased lung epithelial carcinoma cell VEGF (a key growth factor secreted for the vascularization of tumors) synthesis on the 23 nm surface-featured PLGA compared to the nano-smooth substrates for up to 5 days. In summary, these results provided the first insights into understanding the role that PLGA nanotography may play in mediating lung carcinoma cell functions for a wide range of applications in regenerative medicine.
生物材料纳米形貌被认为是一种能显著影响细胞功能（包括成骨细胞、成纤维细胞、内皮细胞、软骨细胞、免疫细胞和细菌）的手段。在这项研究中，系统研究了具有不同纳米形貌的聚乳酸-羟基乙酸共聚物（PLGA）薄膜对肺上皮癌细胞功能（包括粘附（长达 4 小时）、增殖（长达 3 天）、凋亡（长达 5 天）和血管内皮生长因子（VEGF）合成（长达 5 天）的影响。重要的是，本研究制备了具有不同纳米形貌（具体为纳米光滑、23、300 和 400nm 半球形表面特征）但表面化学性质相似的 PLGA 薄膜，以便仅关注 PLGA 形貌对癌细胞功能的影响。采用简单有效的浇铸成型和溶剂蒸发法来实现这一目标。原子力显微镜、化学分析电子能谱和水接触角测量验证了所有本研究中制备的 PLGA 薄膜具有相似的表面化学性质和能量，但具有不同的形貌。肺上皮癌细胞粘附、增殖和形态结果表明，与其他任何样品相比，纳米光滑和 400nm 表面特征的 PLGA 上的细胞生长和扩散较少。然而，结果还表明，与纳米光滑基底相比，23nm 表面特征的 PLGA 上的肺上皮癌细胞 VEGF（一种用于肿瘤血管生成的关键生长因子）合成在长达 5 天的时间内减少。综上所述，这些结果首次深入了解了 PLGA 纳米形貌在调节肺癌细胞功能方面的作用，为再生医学中广泛应用提供了参考。
