Centre International de Recherches Médicales de Franceville, Gabon.
J Infect Dis. 2011 Nov;204 Suppl 3:S800-3. doi: 10.1093/infdis/jir358.
Marburg virus (MARV) nucleic acid was detected in Rousettus aegyptiacus bats in 2005 and 2006 in the midwest and southeast of Gabon. In this study we used MARV-specific real-time reverse-transcription polymerase chain reaction (RT-PCR) and MARV-specific nested RT-PCR assay to screen 1257 bats caught during July 2009, December 2009, and June 2010 in 3 caves situated in northern Gabon. Nine specimens tested positive by the real-time assay, with cycle threshold values ranging from 35 to 39, of which only 1 R. aegyptiacus specimen collected in 2009 was positive in the nested VP35 RT-PCR assay. Together with MARV-positive bats in the south and west found in 2005 and 2006, confirmation of phylogenetically closely related MARV-positive bats 5 years later and in northern Gabon suggests that MARV is now enzootic in Gabon and emphasizes the importance of long-term monitoring of bat populations and human-bat interfaces.
2005 年和 2006 年，在加蓬中西部和东南部，研究人员在埃及果蝠（Rousettus aegyptiacus）中检测到马尔堡病毒（MARV）的核酸。在本研究中，我们使用 MARV 特异性实时逆转录聚合酶链反应（RT-PCR）和 MARV 特异性巢式 RT-PCR 检测方法，对 2009 年 7 月、2009 年 12 月和 2010 年 6 月在加蓬北部的 3 个洞穴中捕获的 1257 只蝙蝠进行了筛查。实时检测方法检测到 9 个样本呈阳性，循环阈值范围为 35 至 39，其中只有 2009 年采集的 1 个埃及果蝠样本在巢式 VP35 RT-PCR 检测中呈阳性。与 2005 年和 2006 年在南部和西部发现的 MARV 阳性蝙蝠一起，5 年后在加蓬北部发现与 MARV 密切相关的阳性蝙蝠，证实 MARV 现在在加蓬流行，并强调了对蝙蝠种群和人与蝙蝠接触界面进行长期监测的重要性。
