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用于血液蛋白质检测的高特异性胺基末端适配体功能化表面等离子体共振生物传感器的研制。

Development of a highly specific amine-terminated aptamer functionalized surface plasmon resonance biosensor for blood protein detection.

作者信息

Zheng Rui, Park Byung-Wook, Kim Dong-Shik, Cameron Brent D

出版信息

Biomed Opt Express. 2011 Sep 1;2(9):2731-40. doi: 10.1364/BOE.2.002731. Epub 2011 Aug 31.

DOI:10.1364/BOE.2.002731
PMID:21991560
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3184881/
Abstract

This paper presents a generally applicable approach for the highly specific detection of blood proteins. Thrombin and thrombin-binding aptamers are chosen for demonstration purposes. The sensor was prepared by immobilizing amine-terminated aptamers onto a gold modified surface using a two-step self-assembled monolayer (SAM) immobilization technique and the physical detection is performed using Surface Plasmon Resonance (SPR). The developed sensor has an optimal detectable range of 5-1000 nM and the results show the sensor has good reversibility, sensitivity and selectivity. Furthermore, the sensor shows the potential of being improved and standardized for direct detection of other blood proteins for clinical applications.

摘要

本文提出了一种普遍适用的高特异性检测血液蛋白质的方法。为了演示目的，选择了凝血酶和凝血酶结合适体。通过使用两步自组装单分子层（SAM）固定技术将胺基末端适体固定在金修饰表面上来制备传感器，并使用表面等离子体共振（SPR）进行物理检测。所开发的传感器具有5 - 1000 nM的最佳可检测范围，结果表明该传感器具有良好的可逆性、灵敏度和选择性。此外，该传感器显示出可改进和标准化以直接检测其他血液蛋白质用于临床应用的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/937c/3184881/68619d60603f/boe-2-9-2731-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/937c/3184881/e037c48157ca/boe-2-9-2731-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/937c/3184881/c3b3ec299262/boe-2-9-2731-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/937c/3184881/750eb1330a43/boe-2-9-2731-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/937c/3184881/69b2d58df5cd/boe-2-9-2731-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/937c/3184881/975e25828f9e/boe-2-9-2731-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/937c/3184881/68619d60603f/boe-2-9-2731-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/937c/3184881/e037c48157ca/boe-2-9-2731-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/937c/3184881/c3b3ec299262/boe-2-9-2731-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/937c/3184881/750eb1330a43/boe-2-9-2731-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/937c/3184881/69b2d58df5cd/boe-2-9-2731-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/937c/3184881/975e25828f9e/boe-2-9-2731-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/937c/3184881/68619d60603f/boe-2-9-2731-g006.jpg

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